Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Organosilicon high-molecular fluorescent probe for detecting hypochlorous acid and preparation method thereof

A technology for the detection of hypochlorous acid and silicon polymers, which is applied in fluorescence/phosphorescence, chemical instruments and methods, analytical materials, etc., to achieve the effects of strong specificity, easy availability of raw materials, and simple synthesis steps

Inactive Publication Date: 2019-01-11
UNIV OF JINAN
View PDF3 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports about fluorescent materi

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Organosilicon high-molecular fluorescent probe for detecting hypochlorous acid and preparation method thereof
  • Organosilicon high-molecular fluorescent probe for detecting hypochlorous acid and preparation method thereof
  • Organosilicon high-molecular fluorescent probe for detecting hypochlorous acid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Synthesis of Fluorescent Probes

[0039] (1) React 50g of 3-aminopropylmethyldimethoxysilane with 50g of dimethyldimethoxysilane to generate aminopropylpolysiloxane under the condition of water as solvent;

[0040] (2) In a 250 mL round bottom flask, add 2 g of aminopropyl polysiloxane, dissolve in 100 mL of ethanol, then add 0.10 g of 4-methylthio-1,8-naphthalene dicarboxylic anhydride, dissolve in 100 mL of Ethanol, heated at 78°C with stirring and reflux for 24h. Rotary evaporation, drying to obtain the compound, i.e. fluorescent probe P-NS-ClO, its 1 H NMR spectrum as figure 1 shown. 1 H NMR (400 MHz, CDCl 3 ) :δ 8.63-8.59 (m, ArH), 8.52 -8.45(m, ArH), 7.76-7.70 (d, J = 12.7 Hz, ArH), 7.47-7.42(m, ArH), 6.46-6.26 (m, ArH), 3.70-3.64 (m, NCH 2 CH 3 ), 2.70-2.62 (t, J = 7.0Hz, NCH 2 ), 2.24-2.01(m, S-CH 3 ), 1.54-1.42 (m, NCH 2 CH 2 ), 1.29-1.19 (m, NCH 2 CH 3 ),0.59 -0.45 (m,Si-CH 2 ), 0.26-0.05 (m, Si-CH 3 ). Its number-average molecular...

Embodiment 2

[0041] Example 2 The selectivity of fluorescent probe P-NS-ClO to different molecules or ions

[0042] Prepare 5 mL of PBS aqueous solutions of various conventional ions and amino acids at a concentration of 40 mM and the fluorescent probe P-NS-ClO mother solution in Example 1 at a concentration of 1 mM as spares.

[0043] Add 25 μL of probe mother solution, 500 μL of ethanol and 500 equivalents of each ion (or amino acid) solution, and dilute to 3 mL with phosphate buffer solution PBS, so that the concentration of the probe is 10 μM, and the concentration of the test ion is 2.5 mM. The concentration of reactive oxygen species and reactive nitrogen species was 100 mM, and the concentration of aldehydes and ketones was 100 μM. Fluorescence detection was performed after shaking for 30 min (λ ex = 405nm,λ em = 485 nm), establish a histogram of fluorescence intensity and each ion (or amino acid, active oxygen, active nitrogen), the results are shown in figure 2 , wherein the ...

Embodiment 3

[0044] Example 3 Fluorescent detection of the fluorescent probe P-NS-ClO to different concentrations of hypochlorous acid

[0045] Prepare 10 mL of an aqueous solution with a concentration of 100 mM hypochlorous acid and a mother solution of the fluorescent probe P-NS-ClO in Example 1 with a concentration of 1 mM as backup.

[0046] Prepare different concentrations of hypochlorous acid (0-20 μM) to react with the probe at a concentration of 10 μM, and perform fluorescence detection (λ ex = 405 nm), calculate the fluorescence intensity in each system, and establish the curve of fluorescence intensity and hypochlorous acid concentration, such as image 3 Shown: As the concentration of hypochlorous acid increases, the fluorescence intensity of the reaction system decreases rapidly, and when the concentration of hypochlorous acid reaches 20 μM, the fluorescence of the reaction system is completely quenched.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an organosilicon high-molecular fluorescent probe for detecting hypochlorous acid. A structural formula is shown as the specification; wherein a, c, and d respectively representthe number of the expressed group monomers; and a, c, and d are respectively positive integers which do not equal 0. The probe can be obtained by reacting aminopropylpolysiloxane and 4-methylthio-1,8-naphthalic anhydride. The probe is capable of detecting the presence of hypochlorous acid in cells with high specificity.

Description

technical field [0001] The invention belongs to the field of organic polymer fluorescent probes, in particular to an organosilicon polymer fluorescent probe and its application in detecting hypochlorous acid. Background technique [0002] Active oxygen is a general term for some oxygen-containing free radicals, light radicals, superoxide anion free radicals, and non-free radicals such as hypochlorous acid and hypochlorous acid that play a very important role in many physiological and pathological processes in organisms. Under physiological and pathological conditions such as oxidative stress and inflammation, organisms produce a variety of enzymes through enzymatic and non-enzymatic reactions. Modern biomedical research shows that active oxygen produced in the body is closely related to the occurrence and development of some diseases and the aging of the body. [0003] Hypochlorous acid is a kind of active oxygen, as an efficient bactericide, it plays an important role in t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C09K11/06G01N21/64C08G77/392C08G77/26C08G77/06
CPCC08G77/06C08G77/26C08G77/392C09K11/06C09K2211/1466G01N21/6428G01N2021/6432
Inventor 林伟英张宇左育静杨婷新王小妮
Owner UNIV OF JINAN
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com