Fluorescence quantitative internal reference gene suitable for Haizhou Changshan flower, primer and application thereof

Abstract

A fluorescence quantitative internal reference gene suitable for Haizhou Changshan flower, its primer and the application thereof are disclosed, wherein the internal reference gene is Actin and AP-2 gene, wherein the gene sequence of the Actin gene is as shown in SEQ ID NO: 1, AP-2 gene is shown in SEQ ID NO: 13. The present application screens out candidate genes with relatively stable expressionaccording to the genes disclosed in the prior art, its stability is evaluated by software, A fluorescence quantitative internal reference gene suitable for Haizhou Changshan flower was obtained, Based on these genes, real-time fluorescence quantitative PCR primers were designed, which filled the situation that there were no internal reference genes in different flowering stages of Haizhou Changshan from different provenances, and provided a powerful support for accurate quantification of related functional genes in different flowering stages of Haizhou Changshan from different provenances, and could improve the stability, repeatability and reliability of the research.

Description

technical field [0001] The invention belongs to the field of plant molecular biology, and specifically relates to an internal reference gene suitable for fluorescence quantification of Haizhou Changshan flower, its primer and application. technical background [0002] Haizhou Changshan (Clerodendrum trichotomum Thunb.), also known as stinky phoenix tree, is a deciduous shrub or small tree of Verbenaceae (Verbenaceae) Daqing genus (Clerodendmm L.). . The drupe is spherical, blue-purple when ripe, shiny, enclosed in the perennial calyx, and does not fall for a long time in autumn and winter. It is a good fruit tree species in autumn and winter, and a rare ornamental tree that combines flower and fruit. The research on the molecular mechanism of Changshan fruit in Haizhou has not been reported yet. According to research, there is no internal reference gene that is constantly expressed under all experimental conditions. The commonly used internal reference genes will show unsta...

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Problems solved by technology

The research on the molecular mechanism of Changshan fruit in Haizhou has not been reported yet. According to research, there is no internal reference gene that is constantly expressed under all experimental conditions. The commonly used internal reference genes will show unstable traits under different plants and experimental conditions, thus Affects the accuracy of target gene detection, so to develop good genes in Changshan, Haizhou, it is necessary to determine stable and reliable internal reference genes

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