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Detection method of ascorbic acid

A detection method, the technology of ascorbic acid, applied in the detection field, can solve problems such as complex operation, high requirements, and great influence on enzyme stability, and achieve the effects of low detection limit, simple operation, good selectivity and anti-interference

Inactive Publication Date: 2019-01-15
ANHUI NORMAL UNIV
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Problems solved by technology

Among them, the enzyme method has too high requirements on the experimental conditions, and the stability of the enzyme has a great influence on the experimental results; gas chromatography cannot be directly used to detect ascorbic acid, and the required pre-treatment work is too cumbersome and the operation is complicated

Method used

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  • Detection method of ascorbic acid
  • Detection method of ascorbic acid
  • Detection method of ascorbic acid

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Embodiment 1

[0030] A detection method for ascorbic acid, comprising the following steps:

[0031] At 25°C, mix 240 μL of 0.5 mM 3,3′,5,5’-tetramethylbenzidine (TMB) solution and 80 μL of 1 mM ceric sulfate solution in a test tube, and add different amounts of ascorbic acid to the test tubes solution, and then add 80 μL of 0.1M citric acid-sodium citrate buffer solution to adjust to PH=5, then add deionized water to make the volume to 1.5mL, and the final concentration of ascorbic acid in the detection system is 0 μM, 1.665 μM, 3.33 μM respectively , 5 μM, 6.67 μM, 8.83 μM, 10 μM, 11.67 μM. Finally, the absorbance was measured using a UV-vis spectrophotometer. After adding ascorbic acid solution to the above system, the blue color of the system gradually becomes lighter, and the degree of lightening is related to the amount of ascorbic acid added. Within a certain concentration range of ascorbic acid, the absorbance of the solution has a linear relationship with the concentration of asco...

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Abstract

The invention provides a detection method of ascorbic acid. The detection method comprises the following steps: Ce(IV) ion can enable 3,3',5,5'-tetramethyl benzidine (TBM) to generate oxidation reaction to produce blue TBM imide; when trace ascorbic acid is added in the Ce(IV) / TMB system, the ascorbic acid generates redox reaction with the TMB imide, so that the blue of the system is shallowed, and the shallowing degree and the concentration of the ascorbic acid are in direct proportion, a linear relation is constructed to realize the detection on the ascorbic acid. Compared with the prior art, the method provided by the invention is simple in operation, can quickly detect the concentration of the ascorbic acid in real time; and the detection limit is low, and can be lowest to 0.08 microns; the detection method has good selectivity and interference resistance.

Description

technical field [0001] The invention belongs to the detection field, and in particular relates to a detection method of ascorbic acid. Background technique [0002] Ascorbic acid is vitamin C, which widely exists in fruits, vegetables, beverages, medicines and the human body. It is one of the important vitamins to maintain the normal physiological functions of the body. However, the human body cannot synthesize it by itself and can only ingest it from the outside. Therefore, the development and design of a A method that can detect low-concentration ascorbic acid has important theoretical and practical values. [0003] At present, the main detection methods of ascorbic acid include catalytic kinetic method, spectrophotometry, chromatography, capillary electrophoresis, enzymatic method, fluorescence method and so on. Among them, the enzyme method has too high requirements on the experimental conditions, and the stability of the enzyme has a great influence on the experimental...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33
CPCG01N21/33
Inventor 孙紫玥刘金水
Owner ANHUI NORMAL UNIV
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