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Biological repairing agent of heavy metal polluted soil and repairing method of heavy metal polluted soil

A bioremediation agent and a technology for polluted soil, which is applied in the field of heavy metal contaminated soil bioremediation agent and its repair, can solve the problems that cannot be applied outdoors in a large area, affect the survival rate of microorganisms, and it is difficult for microorganisms to adapt to the natural environment, so as to improve the survival rate, Avoid secondary pollution and ensure the effect of restoration efficiency

Active Publication Date: 2019-01-18
万物生(深圳)生物科技控股有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the above-mentioned scheme has the following defects: the microorganisms in the above-mentioned scheme are all cultivated under the condition of laboratory medium, so they cannot be applied outdoors on a large scale. If they are directly applied outdoors, the living environment of the microorganisms will change, which may cause the microorganisms to be difficult to adapt. The natural environment affects the survival rate of microorganisms, so it cannot effectively repair the heavy metal soil

Method used

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  • Biological repairing agent of heavy metal polluted soil and repairing method of heavy metal polluted soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A bioremediation agent for soil heavy metal pollution is prepared from the following raw materials according to the percentage of the total mass of raw materials: 5% of biological bacteria stock solution, 1% of antibacterial agent, and the rest is nutrient solution.

[0025] The microorganisms in the biological bacteria stock solution are Rhodobacter sphaericus, and the effective number of viable bacteria in the biological bacteria stock solution is 3 billion / g.

[0026] The components in the nutrient solution are calculated by weight percentage: 64% of distilled water, 6% of lactic acid, 5% of sodium chloride, 10% of peptone, 5% of ammonium phosphate, 8% of glucose and 2% of ascorbic acid.

[0027] Described antibacterial agent is wormwood essential oil.

[0028] At the same time, the invention also discloses a bioremediation method for heavy metal contaminated soil, the specific operation steps are as follows:

[0029] S1: Microbial culture, weigh heavy metal contami...

Embodiment 2

[0034] A bioremediation agent for soil heavy metal pollution is prepared from the following raw materials according to the percentage of the total mass of raw materials: 7% of biological bacteria stock solution, 2% of antibacterial agent, and the rest is nutrient solution.

[0035] The microorganisms in the biological bacteria stock solution are Rhodobacter sphaericus, and the effective number of viable bacteria in the biological bacteria stock solution is 4 billion / g.

[0036] The components in the nutrient solution are calculated by weight percentage: 64% of distilled water, 7% of lactic acid, 5% of sodium chloride, 11% of peptone, 6% of ammonium phosphate, 6% of glucose and 1% of ascorbic acid.

[0037] Described antibacterial agent is lavender essential oil.

[0038] At the same time, the invention also discloses a bioremediation method for heavy metal contaminated soil, the specific operation steps are as follows:

[0039] S1: Microbial culture, weighing heavy metal cont...

Embodiment 3

[0044] A bioremediation agent for soil heavy metal pollution is prepared from the following raw materials according to the percentage of the total mass of raw materials: 8% of biological bacteria stock solution, 2% of antibacterial agent, and the rest is nutrient solution.

[0045] The microorganisms in the biological bacteria stock solution are Rhodobacter sphaericus, and the effective number of viable bacteria in the biological bacteria stock solution is 5 billion / g.

[0046]The components in the nutrient solution are calculated by weight percentage: 54% of distilled water, 10% of lactic acid, 6% of sodium chloride, 15% of peptone, 7% of ammonium phosphate, 7% of glucose and 1% of ascorbic acid.

[0047] Described antibacterial agent is clary sage essential oil.

[0048] At the same time, the invention also discloses a bioremediation method for heavy metal contaminated soil, the specific operation steps are as follows:

[0049] S1: Microbial culture, weighing the heavy meta...

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Abstract

The invention discloses a biological repairing agent of heavy metal polluted soil and a repairing method of the heavy metal polluted soil. The biological repairing agent is prepared from the followingraw materials according to the percentage content of the total mass: 5 to 8 percent of a biological bacterium raw solution, 1 to 2 percent of a bacterium-inhibiting gent and the balance of a nutrientsolution. The biological repairing agent disclosed by the invention is used for treating the heavy metal polluted soil by utilizing microorganisms, secondary pollution to the soil is avoided and thefertility of the soil is not influenced; subsequent crop planting is facilitated; the microorganisms are cultured in the heavy metal soil in advance and the microorganisms adaptive to the soil can beobtained; the potential of hydrogen of the soil is regulated, the microorganisms can be adaptive to a soil environment in a subsequent management process and the survival rate of the microorganisms isimproved, so that the repairing efficiency of the heavy metal polluted soil is ensured.

Description

technical field [0001] The invention belongs to the technical field of soil remediation, and more specifically relates to a bioremediation agent for heavy metal polluted soil and a remediation method thereof. Background technique [0002] Soil is a natural resource for human beings to survive, and it is also the material basis for human beings to obtain food and other renewable resources. With the development of industry and the modernization of agricultural development, soil heavy metal pollution has become a major soil problem. Soil heavy metal pollution has pollutants that move in the soil It has the characteristics of poor performance, long residence time, and cannot be degraded by microorganisms, and can eventually affect human health through media such as water and plants. [0003] For example, the patent application number CN201110122739.6 discloses a microbial repair method for heavy metal-contaminated soil, which specifically includes: Step 1: Separation of Rhodobac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10
CPCB09C1/10B09C2101/00
Inventor 刘永飞马敏戚晓楠
Owner 万物生(深圳)生物科技控股有限公司
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