Method for producing tremella polysaccharide by fermentation and used fermentation medium

A fermentation medium and tremella polysaccharide technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of high cost of culture medium and low yield of tremella polysaccharide, and achieve simple components, low price, The effect of shortening the production cycle

Active Publication Date: 2019-01-18
BLOOMAGE BIOTECHNOLOGY CORP LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of high cost of tremella polysaccharide fermentation medium and low yield of tremella polysaccharide in the prior art, the present invention provides a tremella polysaccharide fermentation medium, which has simple components, low price and high yield of tremella polysaccharide

Method used

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  • Method for producing tremella polysaccharide by fermentation and used fermentation medium
  • Method for producing tremella polysaccharide by fermentation and used fermentation medium
  • Method for producing tremella polysaccharide by fermentation and used fermentation medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Fermentation Medium Nitrogen Content Optimization

[0027] (1) Culture medium preparation:

[0028] Seed medium: peptone 10 g / L, glucose 20 g / L, dipotassium phosphate 1.5 g / L, magnesium sulfate 1.5 g / L and V B1 15 mg / L, pH 6.0.

[0029] Fermentation medium: In this embodiment, four groups of fermentation mediums with different concentrations of corn gluten powder were tested, and the formulations of each fermentation medium were as follows:

[0030] Fermentation medium 1: corn gluten meal 10 g / L, glucose 50 g / L, dipotassium hydrogen phosphate 1.5 g / L, magnesium sulfate 1.5 g / L, pH 6.0;

[0031]Fermentation medium 2: corn gluten meal 7.5 g / L, glucose 50 g / L, dipotassium hydrogen phosphate 1.5 g / L, magnesium sulfate 1.5 g / L, pH 6.0;

[0032] Fermentation medium 3: corn gluten meal 5.0 g / L, glucose 50 g / L, dipotassium hydrogen phosphate 1.5 g / L, magnesium sulfate 1.5 g / L, pH 6.0;

[0033] Fermentation medium 4: corn gluten meal 2.5 g / L, glucose 50 g / L, dipot...

Embodiment 2

[0042] Example 2 Fermentation medium nitrogen source type optimization

[0043] The price of inorganic nitrogen sources is generally lower than that of organic nitrogen sources, therefore, the use of inorganic nitrogen to partially replace the corn gluten meal in Example 1 reduces the cost of the medium. Tremella polysaccharides were produced according to the following method, and the performance of different nitrogen source fermentation media in this example was investigated.

[0044] Seed culture medium and fermentation method, carry out according to embodiment 1.

[0045] The formula of fermentation medium used in the present embodiment is as follows:

[0046] Fermentation medium 1: corn gluten powder 5.0 g / L, glucose 50 g / L, dipotassium hydrogen phosphate 1.5 g / L, magnesium sulfate 1.5 g / L, pH 6.0;

[0047] Fermentation medium 2: corn gluten powder 4.0 g / L, sodium nitrate 1.0 g / L, glucose 50 g / L, dipotassium hydrogen phosphate 1.5 g / L, magnesium sulfate 1.5 g / L, pH 6.0; ...

Embodiment 3

[0055] Example 3 Fermentation Medium Inorganic Nitrogen and Organic Nitrogen Proportion Optimization

[0056] The ratio of inorganic nitrogen source and organic nitrogen source in the fermentation medium was adjusted, and the differences in fermentation days, fermentation broth viscosity, and exosporeal Tremella polysaccharide content were tested under different ratios of inorganic nitrogen source and organic nitrogen source according to the method of Example 2.

[0057] Seed culture medium and fermentation method, carry out according to embodiment 1.

[0058] The fermentation medium formula used in the present embodiment is as follows, and the nitrate is example with sodium nitrate:

[0059] Fermentation medium 1: corn gluten powder 5.0 g / L, dipotassium hydrogen phosphate 1.5 g / L, magnesium sulfate 1.5 g / L, glucose 50 g / L, pH 6.0;

[0060] Fermentation medium 2: corn gluten powder 4.0 g / L, sodium nitrate 1.0 g / L, dipotassium hydrogen phosphate 1.5 g / L, magnesium sulfate 1.5 ...

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Abstract

The invention discloses a method for producing tremella polysaccharide by fermentation and a used fermentation medium. The medium is composed of corn gluten meal, magnesium sulfate, dipotassium hydrogen phosphate, glucose and other effective components. As that composition of the fermentation culture medium is extremely simple. The nitrogen source is preferably provided in the form of a mixture oforganic nitrogen source corn gluten meal and inorganic nitrogen source nitrate. The corn gluten meal is a by-product, and the price is low. Compared with the organic nitrogen sources such as peptoneand yeast powder used in the prior art, the cost is greatly reduced, and is only 30 to 60 percent of the cost of the organic nitrogen source. As that culture medium adopt by the invention is used forferment and producing tremella fuciformis polysaccharide, the yield is high, can reach more than 12.8 g/L, and the production period is shortened, the unit production cost of tremella fuciformis polysaccharide is further reduced, and the tremella fuciformis polysaccharide has important significance in industrial production.

Description

technical field [0001] The invention relates to a fermentation medium required for fermenting and producing white fungus polysaccharide, and also relates to a low-cost and high-yield fermentation production method for tremella polysaccharide by using the fermentation medium, which belongs to the technical field of microbial fermentation and cultivation. Background technique [0002] Tremella polysaccharide has the functions of moisturizing, anti-oxidation, anti-thrombosis, anti-hepatitis, anti-mutation, anti-tumor, improving immunity, lowering blood fat and blood sugar, etc. It is widely used in beauty, medicine, health food and other fields. At present, tremella polysaccharides are mainly prepared by two methods: fruiting body extraction and conidia fermentation. Among them, the fermentation method has the advantages of simple preparation process, low cost, high yield, and high purity of polysaccharides, which has a good development prospect. [0003] In order to obtain hi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12N1/14C12R1/645
CPCC12N1/14C12P19/04
Inventor 郭文逸张天萌孙劭靖郭学平
Owner BLOOMAGE BIOTECHNOLOGY CORP LTD
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