Animal model with jaw defects and construction method and application of animal model

A technology of animal models and jaws, which is applied in animal husbandry and other fields, can solve the problems of large surgical trauma, inability to simulate small clinical jaw defects well, and unfavorable observation of bone regeneration, and achieve the effect of less trauma

Inactive Publication Date: 2019-01-25
HOSPITAL OF STOMATOLOGY SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a result, the bone defect is relatively large, full-thickness penetration, and the bottom is only supported by soft tissue, which is not conducive to the filling and retention of repair materials. Although a thin layer of bone remains at the bottom during preparation, the defect is too shallow and it is difficult to place repair materials. , and this approach is technically difficult to overcome
[0008] 2. There are many important tissue structures in the mandibular angle area of ​​rats, and the surgical trauma is large. The extraoral incision needs to cut off the masseter muscle. At the same time, it is easy to damage the facial nerve, parotid gland, parotid duct, etc., which will affect the postoperative eating of rats, which is not conducive to postoperative rats. recovery, which is neither ethical nor conducive to the observation of bone regeneration
[0009] 3. In addition, the mandibular angle area is far away from the toothed area, which cannot well simulate the clinical situation of small jaw defects
[0012] 1. The posterior teeth of rats are multi-rooted teeth with small roots and bifurcations. The distance from the buccal root surface to the bone surface of rat molars is short, with an average thickness of only 0.40mm. In order to achieve a sufficient depth of bone defect, the posterior The preparation of dental defects often requires amputation of the buccal tooth root, which will expose the pulp cavity, and the defect area will directly penetrate the pulp tissue. The introduction of the pulp tissue will also bring about reactive inflammation, which is not conducive to the observation of jaw regeneration. The study of its mechanism also causes unnecessary suffering to animals and violates experimental ethics
[0013] 2. There are many important tissue structures in the posterior teeth of the mandible in rats. It is necessary to cut off the masseter muscles. At the same time, it is easy to damage the facial nerve and parotid gland duct, which will affect the postoperative eating of rats, which is not conducive to postoperative recovery of rats. It is neither in line with the ethics of animal experiments, nor Not conducive to the observation of bone regeneration
[0014] 3. There are few literature reports on the model of mandibular posterior mandibular defect in rats, and there is a lack of uniform standards. The self-healing of bone defects and the critical value standard have not been reported yet

Method used

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  • Animal model with jaw defects and construction method and application of animal model
  • Animal model with jaw defects and construction method and application of animal model
  • Animal model with jaw defects and construction method and application of animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A kind of embodiment of the jawbone defect animal model of the present invention, described animal model adopts rat as model animal, and the anterior tooth area of ​​rodent has rectangular defect; 2mm.

Embodiment 2

[0045] An embodiment of the construction method of the jaw defect animal model of the present invention comprises the following steps:

[0046] (1) Preparation of experimental materials: syringes of various specifications; surgical instruments such as blades, knife handles, scissors, vascular forceps, sutures, tweezers, etc.; 10% paraformaldehyde (W / W); general anesthesia apparatus for inhalation anesthesia; microCT;

[0047] (2) Experimental animal preparation: prepare healthy rats with a body weight of 300-350 g;

[0048] (3) After the rats are induced anesthetized by inhalation anesthesia general anesthesia apparatus, take the left side lying position of the rat, make the left side of the rat's head face up and fix the body position, prepare the skin in the left mandibular area, and spread the towel after disinfection with iodine;

[0049] (4) Draw a line perpendicular to the lower edge of the mandible at the bone depression on the lower edge of the mandible on the left sid...

Embodiment 3

[0051] Embodiment 3 The effect test of the jaw defect animal model of the present invention

[0052] Test method: Different sizes of rectangular defects (6×2mm, 4×2 mm and 8×2mm) were prepared in the anterior region of the rats, and then observed for 4 weeks before sampling, and the healing of the defect was measured by microCT observation.

[0053] Test result: if figure 2 As shown in the results, it was found that the 4×2mm bone defect rats healed to a greater extent after 4 weeks, while the 6×2mm and 8×2mm bone defect rats still did not heal in a large area after 4 weeks. In order to reduce trauma, the present invention uses a defect model with a size of 6×2mm as the best model. Thus, the animal model of the present invention does not undergo spontaneous complete bone healing within 4 weeks, and can be used to study the ability of the bone repair material to promote bone repair after implantation.

[0054] The animal model of the present invention is suitable for studyin...

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Abstract

The invention discloses an animal model with jaw defects and application of the animal model. A rodent is adopted as the model animal, and a front tooth region of the rodent has the rectangular defects. Meanwhile, the invention discloses a preparation method of the animal model with the jaw defects. According to the animal model with the jaw defects, the non-penetrating bone defects are prepared in the front tooth region of a rat and are close to a dentate region, and regeneration and repair conditions of clinically drilling bone defects can be better simulated; meanwhile, defect preparation wounds are small, there is no need to cut the masseter muscle, and the facial nerve and the parotid duct are not damaged. Through experimental observation, the defects of the animal model with the jawdefects are not completely healed in four weeks, the demands for researching jaw regeneration can be met, and the animal model is a good model with the clinically drilling defects.

Description

technical field [0001] The present invention relates to the construction method of animal model, especially a kind of jaw defect animal model and its construction method and application. Background technique [0002] Craniomaxillofacial bone defects caused by craniomaxillofacial tumors, trauma, congenital developmental defects such as cleft palate, widespread periodontal disease, and alveolar bone resorption after missing teeth are one of the most common clinical symptoms in oral cavity. , has a very high morbidity rate, and seriously affects the normal oral function of patients. Realizing bone regeneration and repair is a key issue in oral clinical practice. [0003] At present, there are many related animal models for bone regeneration repair research, such as skull defect model, femoral defect model, radius defect model, tibial defect model, etc. However, the development of the jaw has its specificities and differs from the histological origin of the bones elsewhere in t...

Claims

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Application Information

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IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor陈泽涛陈卓凡刘冠琪郭远龙张琳珺王小双刘润恒陈首丞林义雄夏彬彬方靖涵
OwnerHOSPITAL OF STOMATOLOGY SUN YAT SEN UNIV