Method for efficiently purifying artemisinin

A kind of artemisinin, high-efficiency technology, applied in the direction of organic chemistry, can solve the problems such as difficult separation of impurities, achieve the effect of reducing recrystallization steps, improving production efficiency and product quality

Active Publication Date: 2019-02-12
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problem that artemisinin is difficult to separate from

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0024] Example 1

[0025] Add 200g of Artemisia annua leaves and petroleum ether (1600mL and 1200mL, corresponding to two extraction processes respectively) into a round bottom flask, reflux extraction at 55℃ for 2 times, extraction time is 2h, combine the two extracts Concentrate the extract at 45°C to obtain an extract containing artemisinin; add 14mL of 70% methanol aqueous solution to the extract, stir and extract at 55°C for 30min, filter after the extraction is complete, add acetic acid to the extract Ethylene-vinyl acetate copolymer with 25% ethylene content and 10% of the mass of the extractant. Stir at 50°C for 2 hours, crystallize at 10°C for 5 hours, separate solid and liquid, and dry at 50°C to obtain crude artemisinin. The artemisinin content is 96%, and the crude product is recrystallized once to obtain an artemisinin product with a purity of 99.2%.

Example Embodiment

[0026] Example 2

[0027] Add 200g of Artemisia annua leaves and petroleum ether (1600mL and 1200mL, corresponding to two extraction processes respectively) into a round bottom flask, reflux extraction at 55℃ for 2 times, extraction time is 2h, combine the two extracts Concentrate the extract at 45°C to obtain an extract containing artemisinin; add 14mL of 70% methanol aqueous solution to the extract, stir and extract at 55°C for 30min, filter after the extraction is complete, add acetic acid to the extract An ethylene-vinyl acetate copolymer with an ethylene content of 40%, the amount of the copolymer added is 10% of the mass of the extractant, stirring at 50°C for 2 hours, crystallization at 10°C for 5 hours, solid-liquid separation, and drying at 50°C to obtain crude artemisinin. The artemisinin content is 93%, and the crude product is recrystallized once to obtain an artemisinin product with a purity of 98.9%.

Example Embodiment

[0028] Example 3

[0029] Add 200g of Artemisia annua leaves and petroleum ether (1600mL and 1200mL, corresponding to two extraction processes respectively) into a round bottom flask, reflux extraction at 55℃ for 2 times, extraction time is 2h, combine the two extracts Concentrate the extract at 45°C to obtain an extract containing artemisinin; add 14mL of 70% methanol aqueous solution to the extract, stir and extract at 55°C for 30min, filter after the extraction is complete, add acetic acid to the extract Ethylene-vinyl acetate copolymer with 25% ethylene content, the amount of copolymer added is 5% of the mass of the extractant, stirred at 23°C for 2 hours, crystallized at 10°C for 5 hours, and dried at 50°C to obtain crude artemisinin with artemisinin content Is 99%.

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Abstract

The invention relates to a method for efficiently purifying artemisinin. The method is characterized in that artemisinin leaching liquor is extracted from leaves of artemisia annua by a leaching solvent and is concentrated to obtain extract; the extract is dissolved by an extracting agent to obtain extraction liquid, and a wax oil inhibitor is added into the extraction liquid to inhibit co-crystallization between wax oil and the artemisinin in the crystallization process, so that the purity of the crude artemisinin is improved; the number of times of recrystallization is reduced, and the production process is simplified. The method has the advantages of simplicity in operation, high purification efficiency, high product purity, recyclability of the inhibitor and the like, and efficient purification and separation of the artemisinin can be realized.

Description

technical field [0001] The invention relates to a method for efficiently purifying artemisinin, which belongs to the field of biochemical industry. Background technique [0002] Artemisinin is a sesquiterpene lactone drug containing peroxy groups extracted from the stems and leaves of the compound inflorescence plant Artemisia annua. The specific antimalarial drug has the characteristics of quick action and low toxicity. Artemisinin is called "the only effective malaria treatment drug in the world" by the World Health Organization. The mechanism of antimalarial action is mainly through the activation of artemisinin to generate free radicals, which bind to malaria protein and act on the membrane structure of Plasmodium, causing damage to the vesicular membrane, nuclear membrane and plasma membrane, mitochondria swelling, and inner and outer membranes falling off , thus causing damage to the Plasmodium cell structure. Studies in recent years have found that artemisinin also...

Claims

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Application Information

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IPC IPC(8): C07D493/20
CPCC07D493/20
Inventor 王慧刘霄张迎张永强张锁江
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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