Heat-resisting type desulphurization bacterium screening culture solution and heat-resisting type desulphurization bacterium screening method
A screening culture and screening method technology, applied in the field of microbiology and environmental engineering, can solve the problems of missed screening of desulfurization bacteria, low content of desulfurization bacteria, slow growth of desulfurization bacteria, etc., and achieve the effect of simple method and good screening effect
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Embodiment 1
[0033] Embodiment 1 Use heat-resistant type desulfurization bacteria screening culture fluid to screen the effect of desulfurization bacteria from hot spring samples
[0034] Add 200mL of heat-resistant desulfurization bacteria screening culture solution into a sterilized 250mL Erlenmeyer flask, add 2mL of hot spring water sample, and cultivate at the temperature corresponding to the hot spring water to screen for desulfurization bacteria.
[0035] The five hot spring water samples are all from Rehai National Geopark, Tengchong County, Yunnan Province, as follows:
[0036] 01: Hot water in the pond downstream of the pregnancy well (70°C when sampling);
[0037] 02: Sulfur pond (above the big boiling pot) hot spring water sample (50°C);
[0038] 03: Water samples from the pond downstream of Hamazui (45°C);
[0039] 04: Cucumber Qing bath water sample (45°C);
[0040] 05: Warm water samples in the ditch outside the Cucumber Qing bathhouse (45°C when sampling)
[0041] The cu...
Embodiment 2
[0046] Embodiment 2 screens the effect of desulfurization bacteria from hot spring sample with other culture fluid
[0047] 1) Screening effect of culture medium with optimized desulfurization bacteria
[0048] The optimized culture medium for screening desulfurizing bacteria is essentially the culture medium without adding auxiliary screening bacteria during the preparation of the high-efficiency screening medium for heat-resistant desulfurizing bacteria.
[0049] Add 200 mL of optimized desulfurization bacteria screening culture solution into a sterilized 250 mL Erlenmeyer flask, add 2 mL of hot spring sample, cultivate at the temperature corresponding to the hot spring water, and carry out screening of desulfurization bacteria. See Example 1 for the hot spring water sample. The cultivation results are shown in Table 2.
[0050] Table 2 Screening results of desulfurization bacteria with optimized desulfurization bacteria screening culture medium
[0051]
[0052] As can...
Embodiment 3
[0066] Embodiment 3 Desulfurization bacteria culture medium adds the screening result of different anaerobic bacillus yellow heat
[0067]In Example 2, the three strains obtained by the research group from the data platform of industrial microbial resources of Chinese universities in Jiangnan University, which can significantly promote the growth of desulfurization bacteria, all belong to Bacillus flavotherii in classification, but the three strains have different colony morphology. The 16s rDNA sequences of the three are also significantly different. Therefore, it is speculated that Bacillus japonica may generally promote the growth of desulfurization bacteria. Accordingly, the inventor purchased two other anaerobic bacillus strains (No.: XQ03734 and XQ03738) from Wuxi Xianqin Biotechnology Co., Ltd. for further experiments. The results showed that the above-mentioned The culture medium obtained by the two strains showed a drop in pH after 4 days of culture after adding hot ...
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