DNA (deoxyribonucleic acid) probe and method for detecting DNA single base mutation

Abstract

The invention belongs to the technical field of molecular detection, and provides a DNA probe and a method for detecting the DNA single base mutation. The sequence of the DNA probe is disclosed by SEQID NO: 1-9; after probe solution is diluted, the probe solution is boiled, annealed and cooled to the room temperature; a mutation target object is added to carry out constant temperature reaction; then, 7Ff-3A and E-Q mixed solution is added; the intensity of the fluorescence spectrum of the mixture is measured, and factor analysis is carried out to obtain a distinguishing result. The DNA probeand the method have the advantages of accuracy and convenience in environment sample detection, good detection method specificity and high sensitivity.

Description

technical field [0001] The invention belongs to the technical field of molecular detection, and in particular relates to a DNA probe and a method for detecting DNA single base mutations. Background technique [0002] DNA single-base mutations, including single-base substitutions, insertions, and deletions, are important biomarkers of human disease and drug resistance. For example, the molecular basis of thalassemia is a single base mutation in the gene sequence on the short arm of chromosome 11, resulting in the failure of normal synthesis of related proteins and inducing thalassemia. [0003] Existing single-base mismatch detection methods include polymerase chain amplification, next-generation sequencing, microarray, and in situ hybridization. These methods have been commercially applied, but they require expensive instruments, complicated operations and highly professional operators, making it difficult to be applied in ordinary laboratories and enterprises. At the same...

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Problems solved by technology

[0004] Aiming at the above technical problems, the present invention provides DNA probes and methods for detecting DNA single base mutations, which solve the problems in the prior art such as complex design of detection methods, expensive instruments, cumbersome professional operations, and high professional requirements for operators.

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