Method for measuring aflatoxin in electronic cigarette solution
A technology of aflatoxin and electronic cigarette liquid, which is applied in the field of tobacco chemical inspection, can solve the problems of low purification efficiency and sensitivity, and achieve high sensitivity, good reproducibility, and the effect of removing matrix interference
Inactive Publication Date: 2019-02-12
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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Problems solved by technology
[0005] The purpose of the present invention is to provide a method for determining aflatoxin in electronic cigarette liquid, so as to solve the problem of low purification efficiency and sensitivity of existing determination methods
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Embodiment 1
[0034] Embodiment 1 of the method for measuring aflatoxin in electronic cigarette liquid of the present invention adopts the following steps:
[0035] 1) Preparation of standard series solutions: using acetonitrile as solvent, prepare a series of mixed standard working solutions of 4 kinds of aflatoxins (aflatoxin B1, B2, G1 and G2) containing isotope internal standard (C13 label); In the working solution, the concentration gradients of the four aflatoxins were all 0.5, 1.0, 2.0, 5.0, 10.0, 20.0 and 50.0 μg / kg, and the concentrations of the four aflatoxin isotope internal standards were all 5.0 μg / kg.
[0036] 2) Preparation of sample extract: add mixed internal standard (the volume of the mixed internal standard solution of 4 kinds of aflatoxin isotopes added is 0.25mL, and the concentration is 500ng / mL), 125mL methanol aqueous solution ( The volume ratio of methanol and water is 7:3) and 5.0g sodium chloride, vortex extraction for 2.0min, and then centrifuge at a speed of 4000...
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Abstract
The invention relates to a method for measuring aflatoxin in an electronic cigarette solution. The method comprises the following steps of 1) adding an extraction solvent and an extraction salt into an electronic cigarette solution sample to perform extraction so as to obtain a sample extracting solution; 2) carrying out solid-phase extraction on the sample extracting solution through a special aflatoxin extraction column, then washing a filling material of the special aflatoxin extraction column after the solid-phase extraction with water, performing desorption by using methanol or acetonitrile, and collecting a desorption solution which is namely a to-be-measured solution; and 3) carrying out liquid chromatography-tandem mass spectrometry on the to-be-measured solution to obtain the aflatoxin. According to the measuring method, the special aflatoxin extraction column is used for performing the solid-phase extraction, the pure water is used for washing the filling material, and the to-be-measured solution is prepared after desorption by methanol; the process is easy in operation, and matrix interference can be effectively removed; the liquid chromatography-tandem mass spectrometry method is adopted to analyze the to-be-measured solution; and the method has the advantages of being good in reproducibility and high in sensitivity, so that rapid and efficient measurement of the aflatoxin in the electronic cigarette solution sample can be realized.
Description
technical field [0001] The invention belongs to the technical field of tobacco chemical testing, and in particular relates to a method for measuring aflatoxin in electronic cigarette liquid. Background technique [0002] Aflatoxins are derivatives of dihydrofuranocoumarins, mainly secondary metabolites produced by Aspergillus flavus, Aspergillus parasitica, and mostly appear in grains, foods and feeds in hot and humid environments. Aflatoxins can damage the liver tissue of humans and animals, and can lead to liver cancer or even death in severe cases. Aflatoxin is classified as a class 1 carcinogen by the World Health Organization Cancer Research Institute, and is a highly toxic and highly toxic substance. Aflatoxins mainly contaminate grain, oil, food, animal and plant foods, etc. Commonly contaminated objects include peanuts, corn, rice, wheat, beans, nuts, meat, milk and dairy products, and aquatic products. At present, there are more than 20 kinds of aflatoxins that ha...
Claims
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Login to View More IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 罗彦波庞永强陈小静朱风鹏姜兴益张洪非李翔宇
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT