A strain of Bacillus subtilis bys2 with high antibacterial properties

A Bacillus subtilis, Escherichia coli technology, applied in bacteria, microorganism-based methods, microorganisms, etc., can solve problems such as harm to human and livestock and poultry health, deterioration of livestock and poultry product quality, and improvement of bacterial resistance levels. Immunity, no toxic side effects, and the effect of maintaining intestinal homeostasis

Active Publication Date: 2021-01-12
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Antibiotics play a key role in the prevention and treatment of bacterial diseases, but with the abuse of antibiotics, it will lead to a series of disadvantages such as the decline in the quality of livestock and poultry products due to drug residues
What's more serious is that the improper use of antibiotics will lead to the continuous increase of the level of bacterial resistance and the continuous expansion of the spectrum of drug resistance, endangering the health of humans and livestock

Method used

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  • A strain of Bacillus subtilis bys2 with high antibacterial properties
  • A strain of Bacillus subtilis bys2 with high antibacterial properties
  • A strain of Bacillus subtilis bys2 with high antibacterial properties

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0033] 1. Obtaining Taishan Bacillus subtilis

[0034] (1) Medium components

[0035] ① Nutrient broth medium (g / L): peptone 10.0, beef extract powder 3.0, sodium chloride 5.0, pH 7.2.

[0036] ② Nutrient agar medium (g / L): peptone 10.0, beef extract powder 3.0, sodium chloride 5.0, agar 15.0, pH 7.3.

[0037] ③ Eosin methylene blue medium (g / L): peptone 10.0, lactose 10.0, dipotassium hydrogen phosphate 2.0, agar 15.0, eosin 0.4, methylene blue 0.065, pH 7.0.

[0038] (2) Isolation of bacterial strains: Take 100 g of soil below 10 cm of the soil surface in four different locations of Mount Tai, put the collected soil into a sterile sterilization bag and bring it back to the laboratory for isolation. Weigh 10 g of the soil in each soil layer and put it into an Erlenmeyer flask containing 100 mL of sterile deionized water, shake for 15 min, and bathe in 80 °C water for 1 h, then add 7 g of NaCl, and bathe in 80 °C water bath for 1 h. Stand still for 10 minutes, absorb 0.5mL ...

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Abstract

The present invention relates to a strain of Bacillus subtilis BYS2 with preservation number CGMCC NO.14220, which has high antibacterial ability; said Bacillus subtilis BYS2 can withstand high temperature, acid and bile salts, and can not only adapt to the environment in the gastrointestinal tract, but also has Escherichia coli, Staphylococcus aureus, and Clostridium perfringens have significant inhibitory effects; Bacillus subtilis BYS2, as a feed additive, can improve the ability of animals to resist pathogenic microorganism infection, that is, disease resistance (p<0.05), and significantly promote Improve the growth performance of broilers and improve the breeding efficiency; at the same time, it has the characteristics of safety, high efficiency and probiotics, and has no toxic and side effects on animals.

Description

[0001] field of invention [0002] The invention relates to a strain of Bacillus subtilis BYS2 with high-efficiency antibacterial performance. [0003] Background of the invention [0004] Bacillus subtilis can secrete substances such as bacteriocins or antimicrobial peptides to inhibit the growth of intestinal pathogenic bacteria such as Escherichia coli, and Bacillus subtilis can decompose starch into simple sugars, synthesize lactic acid through intestinal flora metabolism, and reduce intestinal pH value, and enhance the inhibitory effect on intestinal pathogenic bacteria. Bacillus subtilis also has the function of biological oxygen capture, forming an anaerobic environment in the intestinal tract, which is conducive to the growth of anaerobic beneficial bacteria (lactic acid bacteria, etc.), inhibits the reproduction of pathogenic bacteria, and helps to improve the intestinal micro-ecological environment. Bacillus subtilis can produce amylase, protease and other digestive ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18C12R1/125
CPCA23K10/18C12N1/205C12R2001/125
Inventor 柴同杰郝光恩郭梦娇
Owner SHANDONG AGRICULTURAL UNIVERSITY
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