Breeding method of three-line indica rice male sterile line of wild beating type

A wild-type, indica rice technology, applied in botany equipment and methods, plant gene improvement, application, etc., can solve the problems of poor fertility stability of sterile lines, difficulty in large-scale application, etc., to achieve good grouting and fading, resistance Strong reversibility and tidy heading effect

Active Publication Date: 2022-02-22
江西天涯种业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The second type is the rice red lotus cytoplasm. The maintainer line of this cytoplasmic sterile line is different from the wild type, and the restorer line is also different from the wild type. The above belongs to gametophyte sterile, the pollen is mainly circular abortion, a small amount of fertile pollen will be formed in high temperature climate, the fertility stability of the sterile line is relatively poor
However, there are few early-maturing, high-quality, and high-yielding sterile lines in hybrid rice breeding, and it is difficult to apply them in large-scale production.

Method used

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  • Breeding method of three-line indica rice male sterile line of wild beating type
  • Breeding method of three-line indica rice male sterile line of wild beating type
  • Breeding method of three-line indica rice male sterile line of wild beating type

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The invention provides a method for breeding wild-type three-line indica male sterile lines, comprising the following steps:

[0021] (1) To construct an intermediate indica rice germplasm. In the spring of 2004 at the Sanya Breeding Base in Hainan, the high-quality, blast-resistant three-line restorer germplasm "Guanghui 122" was crossed with the high-quality conventional rice germplasm "Meixiangzhan No. 1". Directed breeding for 4 consecutive generations, bred an intermediate indica germplasm material with early growth period, slender grain shape, and fragrance, named "C195";

[0022] (2) In the spring of 2006, at the Sanya Breeding Base in Hainan, the maintainer line "Yuefeng B" bred by the Rice Research Institute of the Guangdong Academy of Agricultural Sciences was used as the female parent and the "C195" obtained in step (1) was crossed by warming and killing males , the descendants of which were selected and bred for 6 consecutive generations to form a new germpl...

Embodiment 2

[0025] The characteristics of the sterile line Changsheng 843A obtained in Example 1 were tested.

[0026] 1. Sterility

[0027] In the spring of 2017, 20 individual plants of the Changsheng 843A breeding population were inspected under the microscope at the Sanya Breeding Base in Hainan. The pollen sterility of the sterile line reached 100%, and the pollen was typically aborted; the fertility did not fluctuate with changes in the external temperature. The seed setting rate of bagging self-breeding was 0. In August 2017, the group microscopic examination results in Pingxiang, Jiangxi Province showed that 0.02% was stained, 5% was round, and 94.98% was lost. The specific results are shown in Table 1

[0028] Table 1

[0029]

[0030]

[0031] 2. Growth period characteristics

[0032] In the spring of 2017, it was sown in mid-January at the Sanya Breeding Base in Hainan, and the sowing period was 70 days.

[0033] In 2017, the seeds were sown in Pingxiang, Jiangxi on J...

Embodiment 3

[0046] Propagate the sterile line Changsheng 843A obtained in Example 1, as follows:

[0047] For Changsheng 843A in Jiangxi and Hunan, it is better to propagate in autumn. It is sown in mid-to-early June, and the female parent is sown 4-5 days earlier than the male parent. Seed amount for female parent: 37.5kg / hm 2, the amount of seed used in the male parent is 15kg / hm 2 . The breeding field is 1.7m open, and the ratio of male parent to female parent is 2:9. The male parent is planted in small double rows, the density is 16.5cm×23.3cm, and 3-5 seedlings are planted per stalk; the female parent’s transplanting leaf age is controlled within 4.3 leaves for early start, the density is 13.2cm×16.5cm, and each stalk Plant double copies.

[0048] Adopt full health care cultivation, control the amount of nitrogen fertilizer, and increase the application of phosphorus and potassium fertilizers. At the beginning of panicle differentiation, paclobutrazol 1.8kg / hm can be sprayed on ...

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Abstract

The present invention provides a method for breeding wild rot type three-line indica rice sterile line. Firstly, intermediate indica rice germplasm is selected, and then the intermediate indica rice germplasm is crossed with the maintainer line "Yuefeng B" to obtain a new maintainer line germplasm, and then The new germplasm of the maintainer line was crossed with "Shengshi A" to obtain a hybrid germplasm, and finally the hybrid germplasm was backcrossed with the new germplasm of the maintainer line to obtain a backcross sterile line germplasm, and further backcrossed to breed stable sterile Department of germplasm. The invention has the advantages and beneficial effects of being able to obtain indica rice sterile lines with excellent rice quality, strong stress resistance, good combining ability, good outcrossing habit, and large-scale application in production, and good plant and leaf form when compounding and combining , strong tillering ability, more effective ears, tidy heading, good grain filling and color fading, good resistance, high rice quality and high yield.

Description

technical field [0001] The invention relates to the field of hybrid rice breeding, in particular to a method for breeding male sterile lines of indica rice. Background technique [0002] Three-line hybrid rice is a great achievement of rice breeding and promotion. The so-called three-line is: (1) male sterile line: the pistil is normally developed, and the development of the stamen is degraded or aborted, and self-pollination cannot bear fruit; (2) keep Line: the pistil of the male sterile line develops normally, its pollen is given to the pistil of the male sterile line, not only can form seeds, but also can still obtain male sterile plants after sowing; (3) restorer line: its pollen is given to the pistil of the sterile line, After the resulting seeds are sown, the grown plants regain their fertility. [0003] In the three-line hybrid rice breeding technology system, rice cytoplasmic male sterile line breeding and male sterile restorer line breeding are the most basic and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 宋进昌贺浩华杨军邓鹏万根文
Owner 江西天涯种业有限公司
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