Camptosorus sibiricus rupr plant extract with cerebral protection activity and preparation method thereof
A technology of plant extracts and fern fern, applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve problems that have not been reported
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Embodiment 1
[0040] 1. Extraction: 1 kg of Chinese herbal fern, soaked in water and extracted 3 times, each time the water consumption is 12 times of the dry weight of the medicinal material, soaked for 24 hours each time. Combine the extracts, concentrate under reduced pressure to a thick extract, add distilled water to heat and dissolve, cool to room temperature, let stand for 24 hours, and filter to obtain a clear filtrate.
[0041] 2. Alcohol precipitation and refining: extract the concentrated solution of fern fern water, add ethanol (95%) under full stirring, so that the alcohol content of the solution is 70%, let it stand for 24 hours, filter, collect the filtrate, and then recover the ethanol under reduced pressure to liquid extract.
[0042] 3. Macroporous resin adsorption chromatographic purification: dissolve the liquid extract in 2 with water and filter, the filtrate passes through the treated HP-20 type macroporous adsorption resin, water and 30%, 50%, 70%, 95% ethanol Gradie...
Embodiment 2
[0044] 1. Extraction: Add 1 kg of Chinese herbal fern, add water and decoct twice, each time the water consumption is 12 times of the dry weight of the medicinal material, decocting temperature is 98°C, and decocting time is 1 hour each time. Combine the extracts, filter, and concentrate the filtrate under reduced pressure to a thick extract, add distilled water to heat and dissolve, cool to room temperature, let stand for 24 hours, and filter to obtain a clear filtrate.
[0045] 2. Alcohol precipitation and refining: extract the concentrated solution of fern fern water, add ethanol (95%) under full stirring, so that the alcohol content of the solution is 75%, let it stand for 16 hours, filter, collect the filtrate, and then recover the ethanol under reduced pressure to liquid extract.
[0046] 3. Macroporous resin adsorption chromatographic purification: dissolve the liquid extract in 2 with water and filter, the filtrate passes through the treated D-101 type macroporous adso...
Embodiment 3
[0048] 1. Extraction: Add 1 kg of Chinese herbal fern, add water and decoct twice, each time the water consumption is 12 times of the dry weight of the medicinal material, decocting temperature is 98°C, and decocting time is 1 hour each time. Combine the extracts, filter, and concentrate the filtrate under reduced pressure to a thick extract, add distilled water to heat and dissolve, cool to room temperature, let stand for 24 hours, and filter to obtain a clear filtrate.
[0049] 2. Alcohol precipitation and refining: extract the concentrated solution of fern fern water, add ethanol (95%) under full stirring, so that the alcohol content of the solution is 70%, let it stand for 24 hours, filter, collect the filtrate, and then recover the ethanol under reduced pressure to liquid extract.
[0050] 3. Refining by macroporous resin adsorption chromatography: dissolve the liquid extract in 2 with water and filter, pass the filtrate through the treated styrene RH type macroporous ads...
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