Monoclonal Antibody b and Application of Shrimp Hepatopancreatic Pathogenic Toxin Pira

Abstract

The invention relates to a monoclonal antibody B of the pathogenic toxin protein PirA of the prawn hepatopancreas and its application. The monoclonal antibody B is secreted by a hybridoma cell with the preservation number: CCTCC NO: C2017153, and can interact with the epitope of the toxin protein PirA specific binding. The application is to apply the monoclonal antibody B to the pathogen detection of acute hepatopancreatic necrosis in prawns, specifically, to detect whether the prawns contain PirAB plasmid pathogens by using the dot blot method. The monoclonal antibody B developed by the present invention can combine with the PirA protein epitope, so that the monoclonal antibody B can be applied to the early rapid detection of the AHPND pathogen, in order to establish a monoclonal antibody diagnostic method for the AHPND pathogen, and to study the pathogenesis of AHPND It has established a solid foundation for cutting off the infection route, preventing and controlling shrimp AHPND through the infection dynamics, proliferation characteristics, and epidemiological laws.

Description

technical field [0001] The invention belongs to the interdisciplinary technical fields of molecular immunology and pathogenic biology, and relates to a monoclonal antibody and application of a prawn hepatopancreatic pathogenic toxin protein PirA, in particular anti-prawn acute hepatopancreatic necrosis disease (AHPND) pathogenic toxin protein Monoclonal antibody against PirA (Photorhabdus insectrelated A, Photorhabdus insect toxin associated gene A) and its preparation and application. Background technique [0002] Acute hepatopancreatic necrosis (AHPNS) is a disease that has caused a large number of deaths of cultured larvae and juvenile shrimp in Southeast Asia and even the world in recent years. In my country alone, the annual direct economic loss is more than 1 billion US dollars, seriously endangering the development of the shrimp industry, and has caused an unprecedented blow and impact on the global shrimp farming and related industries. For the pathogen of AHPND, th...

AI Technical Summary

Problems solved by technology

Existing molecular biological detection technology requires complex equipment and high cost, and is not suitable for grassroots and on-site detection. Immunological detection technology can form simple and fast on-site detection products. The key to the application of immunological detection technology is to prepare high-quality Antibodies, in recent years, monoclonal antibody technology has been used to improve the authority and specificity of immunological detection technology, making the detection results more reliable

But so far there is no report on the development of monoclonal antibody against shrimp AHPND toxin protein

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