Thallus sample crushing mechanism

Abstract

The invention relates to a thallus sample crushing mechanism, comprising a sample introduction cup, a peristaltic pump, a cooling circulator, a high-pressure homogenizer and a sample collection cup, wherein the cooling circulator is provided with a cold water tank, a sample tube is arranged in the cold water tank, the sample tube is of a spiral structure, the high-pressure homogenizer comprises abody provided with a sample introduction tank and a crushing device, and the sample introduction cup, the peristaltic pump, the sample tube, the sample introduction tank, the crushing device and the sample collection cup are connected in sequence; and a first cold liquid passage is arranged outside the cold water tank, a second cold liquid passage is arranged outside the crushing device, a liquidoutlet of the first cold liquid passage is connected with a liquid inlet of the second cold liquid passage, and a liquid inlet of the first cold liquid passage is connected with a liquid outlet of thesecond cold liquid passage. The thallus sample crushing mechanism has a simple structure and high crushing efficiency, and can ensure the bioactivity of downstream protein of thalli.

Description

technical field [0001] The invention relates to the technical field of cell crushing, in particular to a cell sample crushing mechanism. Background technique [0002] In the prior art, when the BL21-FMDV-B4 cells are broken, the internal temperature of the high-pressure homogenization box is simply reduced, and the temperature of the BL21-FMDV-B4 cells is not substantially reduced. Therefore, the BL21-FMDV-B4 cells A large amount of heat is generated by high-speed impact during the crushing process, causing the downstream proteins of BL21-FMDV-B4 cells to lose their biological activity. In order to reduce the large amount of heat generated during the crushing process of BL21-FMDV-B4 cells, it is necessary to reduce the high-pressure homogenization The crushing pressure of the homogenizer reduces the crushing efficiency of the high-pressure homogenizer. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a bacterial ...

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Problems solved by technology

[0002] In the prior art, when the BL21-FMDV-B4 cells are broken, the internal temperature of the high-pressure homogenization box is simply reduced, and the temperature of the BL21-FMDV-B4 cells is not substantially reduced. Therefore, the BL21-FMDV-B4 cells A large amount of heat is generated by high-speed impact during the crushing process, causing the downstream proteins of BL21-FMDV-B4 cells to lose their biological activity. In order to reduce the large amount of heat generated during the crushing process of BL21-FMDV-B4 cells, it is necessary to reduce the high-pressure homogenization The crushing pressure of the homogenizer reduces the crushing efficiency of the high-pressure homogenizer

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