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Method for artificial culture of selenium-enriched nostoc sphaeroides

A kind of Gexian rice, selenium-enriched technology, applied in the field of aquatic plant cultivation

Inactive Publication Date: 2019-03-01
恩施硒谷科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The organic selenium enrichment of Gexianmi to selenium benefits from its protein content of about 56%, and there are few reports on the research on selenium-enriched Gexianmi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for artificially cultivating selenium-enriched Ge Xianmi, the steps are:

[0022] (1) Configuration of culture medium: Culture medium composition: sodium bicarbonate 35mg / L, disodium hydrogen phosphate 80mg / L, citric acid 6mg / L, ferric ammonium citrate 5mg / L, calcium chloride 210mg / L, magnesium sulfate 70mg / L, boric acid 1.7mg / L, manganese chloride 1.6mg / L, zinc sulfate 0.4mg / L, sodium molybdate 0.3mg / L, copper sulfate 0.07mg / L, cobalt nitrate 0.05mg / L.

[0023] (2) Stability of the culture medium: put the prepared culture medium into the ultrasonic board, 127 Hz, pressurize 10Mpa after ultrasonication, and maintain on the ultrasonic board for 30min.

[0024] (3) activation of the original species: get the prepared nutrient solution, and inoculate the Gexianmi bacterial classification at a proportion of 3.6‰ (the Gexianmi bacterial classification in this embodiment is collected from Zouma Town, Hefeng, and can also be used in other embodiments. Select Gexianmi...

Embodiment 2

[0029] A method for artificially cultivating selenium-enriched Ge Xianmi, the steps are:

[0030] (1) Configuration of culture medium: Culture medium composition: sodium bicarbonate 35mg / L, disodium hydrogen phosphate 80mg / L, citric acid 6mg / L, ferric ammonium citrate 5mg / L, calcium chloride 210mg / L, magnesium sulfate 70mg / L, boric acid 1.7mg / L, manganese chloride 1.6mg / L, zinc sulfate 0.4mg / L, sodium molybdate 0.3mg / L, copper sulfate 0.07mg / L, cobalt nitrate 0.05mg / L.

[0031] (2) Stability of the culture medium: Put the prepared culture medium into the ultrasonic board, 127 Hz, pressurize 10Mpa after ultrasonication, and maintain it on the ultrasonic board for 10 minutes.

[0032] (3) Activation of the original species: Take the prepared culture medium and inoculate the Gexian rice strain (the Gexian rice strain is collected from the wild strain in Zouma Town, Hefeng) at a proportion of 3.6‰, and the temperature is controlled at 19.7°C , into pure CO 2 Incubate for 1.8 hou...

Embodiment 3

[0037] A method for artificially cultivating selenium-enriched Ge Xianmi, the steps are:

[0038](1) Configuration of culture medium: Culture medium composition: sodium bicarbonate 35mg / L, disodium hydrogen phosphate 80mg / L, citric acid 6mg / L, ferric ammonium citrate 5mg / L, calcium chloride 210mg / L, magnesium sulfate 70mg / L, boric acid 1.7mg / L, manganese chloride 1.6mg / L, zinc sulfate 0.4mg / L, sodium molybdate 0.3mg / L, copper sulfate 0.07mg / L, cobalt nitrate 0.05mg / L.

[0039] (2) Stability of the culture medium: put the prepared culture medium into the ultrasonic board, 127 Hz, pressurize 10Mpa after ultrasonication, and maintain on the ultrasonic board for 30min.

[0040] (3) Original species activation: get the prepared nutrient solution, and inoculate the Gexian rice strain (the Gexian rice strain is collected from Zouma Town, Hefeng, and can also be selected from other sources of Pueraria spp. rice, not limited to specific strains), the temperature was controlled at 19.7...

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Abstract

The invention discloses a method for artificial culture of selenium-enriched nostoc sphaeroides. The method comprises the steps: (1) preparation of a culture solution: preparing the culture solution from sodium bicarbonate, disodium hydrogen phosphate, citric acid, ferric ammonium citrate, calcium chloride, magnesium sulfate, boric acid, manganese chloride, zinc sulfate, sodium molybdate, copper sulfate and cobalt nitrate in a certain ratio; (2) stabilization of the culture solution: placing an ultrasonic plate in the culture solution, carrying out ultrasonic treatment, then, carrying out pressurizing, and carrying out ultrasonic treatment; (3) activation of stock seeds: inoculating the culture solution with nostoc sphaeroides inocula, and introducing pure CO2 for culture, so as to obtainan activated inoculum solution of the nostoc sphaeroides; (4) inoculated culture: weighing the activated inoculum solution of the nostoc sphaeroides, controlling intensity of illumination during culture, and carrying out illumination offering according to natural daytime for culture; (5) selenium-enriching culture: after culture, adding sodium selenite, and carrying out culture under the conditionof natural light; and (6) harvesting: carrying out filtering, taking out nostoc sphaeroides, and carrying out ultrasonic cleaning with ultrapure water, thereby obtaining the selenium-enriched nostocsphaeroides. The selenium content is 350ppm to 2,000ppm, the diameter of the nostoc sphaeroides reaches 0.8cm to 1.4cm, the protein content reaches 52% or more, and the nutritive value is high.

Description

technical field [0001] The invention belongs to the field of aquatic plant cultivation, and more specifically relates to a method for artificially cultivating selenium-enriched kudzu rice. Background technique [0002] Gexianmi (Latin name: Pogostemon auricularius (L.) Kassk; scientific name: Pseudosphaeroides) is commonly known as Tianxian rice, Tianxian vegetable, pearl vegetable, water fungus, and field fungus. Gexianmi Cyanobacteria, Nostocaceae. The algae are colloidal, spherical or other irregular shapes, blue-green or yellowish-brown. The cells are spherical, and the majority of cells are connected into a bead-like group, and the outsourcing is colloid. Its total protein is as high as about 56%, crude fat 8.11%, carbohydrate 12.69%, ash content 10.88%, chlorophyll 30.98mg / g, ascorbic acid 5.50mg / g, vitamin C content close to that of fresh jujube, more than 5 times higher than hawthorn, higher than orange Orange is 5 times higher; Vitamin B 1 , B 2 Higher than ord...

Claims

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Application Information

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IPC IPC(8): A01G33/00C05G1/00
CPCA01G33/00C05B7/00C05G5/20C05C3/00C05C5/00C05D3/00C05D5/00C05D9/00C05D9/02C05F11/00
Inventor 彭祚全余爱民朱于鹏
Owner 恩施硒谷科技股份有限公司
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