Tissue culture method of two-step seedling formation of Yanhuanglian

A Coptis chinensis and tissue culture technology, applied in the field of plant seedlings, can solve the problems of shortening the cultivation cycle, etc., and achieve the effects of shortening the cultivation time, large growth of stems, leaves and roots, and high activity

Active Publication Date: 2021-08-24
GUANGXI MEDICAL UNIVERSITY +1
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  • Claims
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Problems solved by technology

However, the whole cycle still takes 2-3 months, and the cultivation cycle and cultivation cost still need to be studied to shorten the cultivation cycle, reduce the cultivation cost, and select the best cultivation method

Method used

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  • Tissue culture method of two-step seedling formation of Yanhuanglian
  • Tissue culture method of two-step seedling formation of Yanhuanglian
  • Tissue culture method of two-step seedling formation of Yanhuanglian

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Embodiment 1

[0025] The two-step seedling tissue culture method of Yanhuanglian comprises the following steps:

[0026] (1) Disinfection of explants: Take the young and healthy leaves of Litectinus chinensis as explants, wash them twice with tap water, absorb the surface moisture with filter paper, put them in 75% alcohol for disinfection for 30 seconds, wash them twice with sterile water, and wash them twice with sterile water. Dry the surface moisture on the paper, transfer to 0.1% mercuric chloride solution for disinfection for 2 minutes, wash with sterile water 5 times, and dry the surface moisture with sterile paper.

[0027] (2) Explant pretreatment: cut off the margin and petiole of the sterilized leaves, and then cut the remaining part of the leaves into small square pieces with a size of 5mm×5mm.

[0028] (3) Induction cultivation: transfer the small leaves obtained in step (2) into the induction medium and cultivate them for 40 days to induce adventitious buds. The cultivation co...

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Abstract

The invention discloses a two-step seedling tissue culture method of the traditional Chinese medicine Litectin Rhizoma. In the method, the leaves of Litectin Rhizoma are used as explants, and the tissue culture seedlings of Litectin Rhizoma can be obtained only through two steps of induction cultivation and rooting cultivation. The tissue culture saves a process. The method of the invention can provide a large amount of high-quality seedlings of Litectin chinensis in a short period of time, reduces the steps of preparing culture medium and turning bottles, saves a lot of manpower and material resources, and is therefore simple and easy to implement and has low production cost. Moreover, the method of the present invention screens out the best planting technology for the tissue culture of the rock berthia, and lays a foundation for establishing the industrialization standard of the rock berthia tissue culture technology.

Description

technical field [0001] The invention belongs to the technical field of plant seedling cultivation, and in particular relates to a two-step seedling-forming tissue culture method of the traditional Chinese medicine rock berberine. Background technique [0002] Rock berth ( Corydalis saxicola Bunting ) is a perennial herb of the Corydalaceae Corydalis genus. It is mostly found in the mountainous areas of Northwest Guangxi in Guangxi. It naturally grows on rocky cliffs or alpine caves. The growth temperature is between 15°C and 25°C. Because of its harsh growth conditions, it is distributed regionally. Narrower, Donglan, Bama, Du'an, Jingxi, and Debao are more common. It has become a common Chinese herbal medicine used by local people for anti-inflammatory and pain relief, detoxification, treatment of scabies and swelling, and treatment of hepatitis, cirrhosis and other diseases. The whole herb of Litectin contains active ingredients such as dehydrocarvitin (Rectine), which ha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 朱丹王德宝康亮郭宏伟苏志恒
Owner GUANGXI MEDICAL UNIVERSITY
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