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Composition for detecting human CYP3A4 gene polymorphism, kit, sample processing method and application

A technology for gene polymorphism and detection reagents, applied in the fields of biomedicine and nucleic acid detection, can solve the problems of increasing operation steps, increasing false positive rate and false negative rate, time-consuming and laborious, etc.

Inactive Publication Date: 2019-03-01
湖南健基生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These techniques must go through tedious nucleic acid extraction and purification processes to obtain higher purity template DNA
Increase the operation steps, time-consuming and labor-intensive, and increase the false positive rate and false negative rate caused by human error

Method used

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  • Composition for detecting human CYP3A4 gene polymorphism, kit, sample processing method and application
  • Composition for detecting human CYP3A4 gene polymorphism, kit, sample processing method and application
  • Composition for detecting human CYP3A4 gene polymorphism, kit, sample processing method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Example 1 sample detection (20070 heterozygotes, 20230 homozygotes)

[0078] Using the above method to detect the sample, the results are as follows: figure 1 As shown, the genotype is 20070TC, 20230GG.

Embodiment 2

[0079] Example 2 Sample detection (20070 homozygotes, 20230 heterozygotes)

[0080] Using the above method to detect the sample, the results are as follows: figure 2 As shown, the genotype of the sample is 20070TT, 20230GA.

Embodiment 3

[0081] Example 3 Sample detection (20070 homozygotes, 20230 homozygotes)

[0082] Using the above method to detect the sample, the results are as follows: image 3 As shown, the genotype of the sample is 20070TT, 20230GG.

[0083] Hunan Jianji Biotechnology Co., Ltd.

[0084] A composition, kit, sample processing method and application for detecting human CYP3A4 gene polymorphism

[0085] 8

[0086] 1

[0087] 23

[0088] DNA

[0089] Artificial sequence

[0090] 1

[0091] ccactcaccctgatgtccagcag 23

[0092] 2

[0093] 27

[0094] DNA

[0095] Artificial sequence

[0096] 2

[0097] ctcttcatctaaactgtgatgccctac 27

[0098] 3

[0099] 19

[0100] DNA

[0101] Artificial sequence

[0102] 3

[0103] cagctctgtccgatctgga 19

[0104] 4

[0105] 19

[0106] DNA

[0107] Artificial sequence

[0108] 4

[0109] cagctctgtccgatccgga 19

[0110] 5

[0111] 25

[0112] DNA

[0113] Artificial sequence

[0114] 5

[0115] catagccagcaaaaataaagataat 25

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PUM

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Abstract

The invention relates to a composition for detecting human CYP3A4 gene polymorphism, a kit, a sample processing method and application. The composition comprises primers for detecting the polymorphismof 20070 locus and 20230 locus of a human CYP3A4 gene, wherein the primers for detecting the polymorphism of the 20070 locus of the human CYP3A4 gene comprise primers with the sequence shown in SEQ ID NO.1 and SEQ ID NO.2, and primers for detecting the polymorphism of the 20230 locus of the human CYP3A4 gene comprise primers with the sequence shown in SEQ ID NO.5 and SEQ ID NO.6. The primers of the invention do not interfere mutually, and have high specificity, good sensitivity and repeatability.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid detection in clinical detection technology in the field of biomedicine, and specifically relates to a composition, a kit, a sample processing method and an application for detecting human CYP3A4 gene polymorphism. Background technique [0002] Polymerase chain reaction (PCR) technology is one of the most commonly used technologies in current nucleic acid detection. Among them, real-time fluorescent PCR technology using fluorescently labeled taqman probe method has been widely used in nucleic acid detection, clinical diagnosis and molecular biology research. Very mature, this technology has been applied to many industries such as laboratory research, food safety, medicine and health, but most of these technologies use a single reaction tube to detect a single target nucleotide, when multiple target nucleotides are detected in a single reaction tube Because primers and probe sequences for detec...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 李仁君文荻琛罗哲容覃武明
Owner 湖南健基生物技术有限公司