Compositions and methods for improving plant development
A technology for plant development and composition, applied in the field of compositions and methods for improving plant development, can solve problems such as affecting microbial vitality, changing greatly, affecting plant yield and growth, etc.
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Embodiment 1
[0066] 1-1 / Preparation of bacterial strain inoculum: Delftia acidophilus
[0067] The expected bacterial concentration in each inoculum was estimated to be equivalent to 4 10 11 CFU / m 3 A commercially available strain of Delftia acidovorans, labeled LPC8. Since the volume of the pot used during the test was 0.0003m 3 , so it is determined that each pot needs to add 1.2·10 8 CFU.
[0068] 1-2 / Counting techniques for bacterial suspensions:
[0069] The bacterial strain Delft acidophilus RAY209 is sold in liquid form (BioBoost Liquid or BBL) containing a suspension of the bacteria in its medium. To understand the bacterial concentration of the BBL, the bacteria in the product were counted. From the initial Bag-in-Box TM (BBL) 1 ml of the bacterial suspension was removed and placed in a test tube. Peptone water (9ml) was added to the test tube containing 1ml of bacterial suspension for 10 -1 dilution. Put 1 ml of this dilution into the test tube as a second sample,...
Embodiment 2
[0086] 2-1 / Plant preparation (Arabidopsis thaliana)
[0087] Plants were grown on solid medium under non-sterile conditions to approximate natural conditions in the field according to the following repetitions:
[0088] Biological replicates = 8 protocols x 3 replicates x 6 iterations = 144 plants
[0089] 2-2 / Preparation of seedlings
[0090] Seeds were stored at 4°C to ensure properly synchronized germination (ABRC, 2015). Approximately 300 to 400 seeds were placed sparsely on germination paper in 3 petri dishes (14 cm diameter). A 5ml equivalent of water was added to briefly soak the sprouting paper. The dishes were placed in a refrigerator (4°C) for 3 days to ensure seed stratification.
[0091] 2-3 / Preparation of Micro Greenhouses and Pots
[0092] Plants were grown in pots (6 cm x 6 cm x 7 cm) placed in 6 microgreenhouses (22 cm x 16 cm x 18 cm). The pots were charged with 150 g of a breeding soil-sand mixture (2 / 3 breeding soil and 1 / 3 sand, mass / mass).
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Embodiment 3
[0115] Effects of inactivation of Delftia and Lactobacillus strains on growth parameters of rape seedlings
[0116] The purpose of this study was to determine the effect of pasteurization treatment with strains of Delftia acidovorans and Lactobacillus rhamnosus on the growth of rape seedlings (B. napus variety 5525CL). More specifically, the study sought to compare the effects of pasteurized and unpasteurized bacterial strains (ie, live bacterial cultures) on the growth parameters of canola seedlings.
[0117] 3-1 / Rape varieties
[0118] ("Metabolomics Differentiation of Canola Genotypes: Toward an Understanding of Canola Allelochemicals." Frontiers in Plant Science, Vol. 5, 2015. doi: 10.3389 / fpls.2014.00765) European The rape seedlings of rapeseed are sterilized. After drying, the seeds were germinated in petri dishes containing agar (15 g / l). Germinated seeds were transferred at 2 seeds / bag to germination bags (Mega International) containing a breeding soil mix supple...
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