Pseudomonas strain and identification and application methods thereof

A technology of pseudomonas and strains, applied in the field of microorganisms, can solve problems such as long cycle

Active Publication Date: 2019-03-05
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, there are two common detection and identification methods for Pseudomonas: 1. Physiological and biochemical identification, through the isolated pure bacteria, qualitative research on the microbial physiology and biochemistry, this method has a long cycle and p

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  • Pseudomonas strain and identification and application methods thereof
  • Pseudomonas strain and identification and application methods thereof
  • Pseudomonas strain and identification and application methods thereof

Examples

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Embodiment 1

[0085] Example 1: Using Pseudomonas SINOPEC21 to identify soil samples above oil and gas reservoirs.

[0086] (1) Design four pairs of specific primers according to the 16S rDNA conserved sequence of Pseudomonas SINOPEC21, respectively:

[0087] Upstream primer 1: 5'-GCGACCACCTGGACTGATACTGACACTG-3',

[0088] Downstream primer 1: 5'-AACCCAACATCTCACGACACGAGCTGAC-3';

[0089] Upstream primer 2: 5'-GTTAATCGGAATTACTGGGCGTAAAGCG-3',

[0090] Downstream primer 2: 5'-AACGTGCTGGTAACTAAGGACAAGGGTT-3';

[0091]Upstream primer 3: 5'-TCAGCGGCGGACGGGTGAGTAATGCCTAG-3',

[0092] Downstream primer 3: 5'-TGCGCTCGTTACGGGACTTAACCCAACA-3';

[0093] Upstream primer 4: 5'-AGCTTGCTCCTTGATTCAGCGGCGGACG-3',

[0094] Downstream primer 4: 5'-GATTAGCTCCACCTCGCGGCTTGGCAAC-3'.

[0095] Wherein, the size of the target band amplified by primer 1 is 361bp, the size of the target band amplified by primer 2 is 588bp, the size of the target band amplified by primer 3 is 1012bp, and the size of the target ba...

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Abstract

The invention relates to a pseudomonas strain and identification and application methods thereof in the technical field of microorganisms. The rhodococcus strain has a 16S rDNA (16S ribosome deoxyribonucleic acid) gene sequence shown as SEQ ID NO.1, a strain name of SINOPEC21 and a preservation number of CCTCC NO: M2016111 in China Center for Type Culture Collection. The abundance of the pseudomonas strain in soil above an oil reservoir is positively related to the concentration of floating gaseous hydrocarbons of the oil reservoir, so that the pseudomonas strain can serve as an oil exploitation microorganism to mark areas with high hydrocarbon leakage above the oil reservoir; meanwhile, the pseudomonas strain is high in abundance in soil above the oil reservoir and saves amplification culture, the 16S rDNA conserved sequence of the pseudomonas strain SINOPEC21 in soil through improved primers, so that the concentration of floating gaseous hydrocarbons of the oil reservoir can be determined accurately and efficiently, and the deficiency of long detecting cycle and high requirements on purity of sample culture in traditional physiological and biochemical detection can be solved.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a pseudomonas and its identification method and application. Background technique [0002] The core of microbial exploration technology is the identification of oil and gas indicator microorganisms. Usually, the relative abundance of the target microorganisms studied in the exploration process is very low in the environment, so it is necessary to stimulate the rapid reproduction of hydrocarbon oxidizing bacteria in the oil and gas reservoir environment and non-oil and gas reservoir environment under the traditional specific culture conditions. Although the above method can obtain the quantity information of microorganisms, the quantity information is actually geometrically higher than the number of microorganisms in the in-situ environment, which inevitably conceals the difference in the number of microorganisms in oil and gas reservoirs and non-oil and gas reservoir...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/64C12Q1/04C12R1/38
CPCC12Q1/64C12Q1/689C12R2001/38C12N1/205Y02W10/10
Inventor 顾磊许科伟汤玉平杨帆宁丽荣
Owner CHINA PETROLEUM & CHEM CORP
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