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Preservation of Bone Specimens by Using Vacuum Impregnation Technology

A vacuum impregnation and specimen technology, which is applied in the preservation, application, animal husbandry and other directions of human or animal body, can solve the problems of unsuitable protection of bone specimens, unsolved bone specimens, uneven paint film, etc., to achieve good protection effect, The effect of improving utilization and high transparency

Active Publication Date: 2020-04-24
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] CN201410599448.X discloses a method and device for preserving animal / plant tissues, cells, organs and body fluids, by placing the sterilized animal / plant tissues, cells and organs in the same sterilized preservation Afterwards, the storage device is placed in a freeze dryer for freeze-drying. After the freeze-drying, the storage device is vacuumized, and the discharge port of the storage device is hot-melt-sealed while the storage device is kept in a vacuum state. The upper end of the discharge port of the container and the sealing plug are removed by wire drawing, and the container becomes a sealed whole, and then the container is stored for a long time; the animal / plant body cells, tissues and organs are sealed after freeze-drying, so that the animal / plant body Somatic cells, tissues and organs are isolated from the outside air to facilitate storage at room temperature, which greatly reduces the preservation conditions and costs of animal / plant somatic cells, tissues and organs, but it is not applicable to the protection of bone specimens, and there is no solution to bone specimens. The paint film is too thick and the paint film is uneven

Method used

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  • Preservation of Bone Specimens by Using Vacuum Impregnation Technology
  • Preservation of Bone Specimens by Using Vacuum Impregnation Technology
  • Preservation of Bone Specimens by Using Vacuum Impregnation Technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Prepare the bone samples before immersion and put them in an oven to fully dry for 20 minutes. The temperature is kept at about 100°C, so as to discharge the air and moisture in the bone as much as possible. At the same time, brush the container with simethicone to prevent the glue from sticking to the container. Mix HZ-806A and HZ-806B in a mass ratio of 3:1 to prepare a two-component epoxy epoxy working solution. Mix glycerol and ethanol at a volume ratio of 2:1 to prepare a protective solution, and place it in a sealed container to prevent moisture for later use.

[0057] Vacuum impregnation and curing Take the sample out of the oven and immerse it in a container containing the prepared working solution while it is hot. For the long bones of the limbs, etc., make the working solution cover the bone end by about 1cm, and keep the backbone and the other end of the bone in the air. middle. Afterwards, the container is placed in the vacuum chamber, and the vacuum pump ...

Embodiment 2

[0060] Prepare the bone samples before immersion and put them in an oven to fully dry for 20 minutes. The temperature is kept at about 100°C, so as to discharge the air and moisture in the bone as much as possible. At the same time, brush the container with simethicone to prevent the glue from sticking to the container. Mix HZ-806A and HZ-806B in a mass ratio of 3:1 to prepare a two-component epoxy epoxy working solution. Mix glycerol and ethanol at a volume ratio of 2:1 to prepare a protective solution, and place it in a sealed container to prevent moisture for later use.

[0061] Vacuum impregnation and curing Take the sample out of the oven and immerse it in a container containing the prepared working solution while it is hot. For bones other than the long bones of the limbs, wrap them with wire mesh or gauze and completely immerse them in the working solution. Place the container in the vacuum chamber afterwards, turn on the vacuum pump, start timing after the air pressur...

Embodiment 3

[0063] Prepare the bone samples before immersion and put them in an oven to fully dry for 20 minutes. The temperature is kept at about 100°C, so as to discharge the air and moisture in the bone as much as possible. At the same time, brush the container with simethicone to prevent the glue from sticking to the container. Mix HZ-806A and HZ-806B in a mass ratio of 3:1 to prepare a two-component epoxy epoxy working solution. Mix glycerol and ethanol at a volume ratio of 2:1 to prepare a protective solution, and place it in a sealed container to prevent moisture for later use.

[0064] Vacuum impregnation and curing Take the sample out of the oven and immerse it in a container containing the prepared working solution while it is hot. For the long bones of the limbs, etc., make the working solution cover the bone end by about 1cm, and keep the backbone and the other end of the bone in the air. middle. Afterwards, the container is placed in the vacuum chamber, and the vacuum pump ...

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Abstract

The invention provides a method for protecting bone specimens by a vacuum impregnation technique. The method includes the following steps: soaking pretreated bone samples in a working fluid, performing vacuum decompression to the 5% standard atmospheric pressure, and soaking the bone samples until bubbles disappear; soaking the treated bone samples in a protecting liquid until the interiors and the surfaces of bones are initially cured with paint films; subjecting the treated bone samples to desolventizing and dry curing to obtain the bone specimens. The method has the advantages that the paint films on the surfaces of the bone specimens are thin and uniform, and the bone specimens are protected internally and externally, so that the service life of the bone specimens is prolonged, and theshortage of remains resources is alleviated.

Description

technical field [0001] The invention relates to the technical field of specimen protection, in particular to a method for protecting bone specimens by adopting vacuum impregnation technology. Background technique [0002] In the context of few sources of remains and low reuse rate in my country, the protective treatment of bone specimens is of great significance, but there are few related studies, and the protection method is extremely single. The more common method is to paint the outer surface of the specimen with polyester paint before stringing to prevent mildew and maintain appearance. However, this method can only protect the surface of bone specimens to a certain extent, and has limited protection effect on some specimens whose damage rate is increased due to low bone density, and the paint film is too thick and uneven, easy to change color, and volatiles are harmful. Fractures and other drawbacks, and the protection of the prior art for bone specimens is limited to ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/00
CPCA01N1/00
Inventor 潘爱华李青耘张齐胡建光戴家富萨达木·玉散王森文楚琦赵子睿
Owner CENT SOUTH UNIV
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