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Enzyme-based composite nanoprobe and its preparation method and application

A nanoprobe and nanoparticle technology, which is applied in the field of enzyme-based composite nanoprobes and its preparation, can solve problems such as limited penetration depth, and achieve the effects of reducing background interference, efficient detection, and stable performance

Active Publication Date: 2022-04-08
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the above-mentioned deficiencies of the prior art, provide an enzyme-based composite nanoprobe and its preparation method and application, to solve the problem of photon penetration in the body when the existing optical probe is used to detect D-amino acids in the body Depth limited bottleneck technical issues

Method used

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  • Enzyme-based composite nanoprobe and its preparation method and application
  • Enzyme-based composite nanoprobe and its preparation method and application
  • Enzyme-based composite nanoprobe and its preparation method and application

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preparation example Construction

[0029] On the other hand, the embodiment of the present invention also provides a preparation method of the enzyme-based composite nanoprobe. The preparation method process of the enzyme-based composite nanoprobe is as follows: figure 2 and 3 As shown, it includes the following steps:

[0030] Step S01: mixing the oleic acid-coated upconverting nanoparticles with the amphiphilic polymer and separating and purifying them to obtain water-soluble upconverting nanoparticles;

[0031] Step S02: mixing the water-soluble upconversion nanoparticles with oxygen-sensitive fluorescent molecules, separating and purifying them, to obtain upconversion nanoparticles carrying oxygen-sensitive fluorescent molecules;

[0032] Step S03: Mixing the upconversion nanoparticles carrying oxygen-sensitive fluorescent molecules with D-amino acid oxidase and performing covalent coupling reaction treatment to obtain enzyme-based composite nanoprobes.

[0033] Wherein, in the step S01, during the mixi...

Embodiment 1

[0045] This embodiment provides an enzyme-based composite nanoprobe and its preparation method. The structure of the enzyme-based composite nanoprobe is as follows figure 1 As shown, it is a core-shell structure, the particle size of the core body is 20nm, and the core body includes NaYF 4 , the doping elements are Er and Yb; the shell material includes an oleic acid layer and a C18-PEG-COOH amphiphilic polymer combined with the oleic acid layer, and an oxygen-sensitive fluorescent molecule platinum octaethyl is assembled in the shell Porphyrin and D-amino acid oxidase groups.

[0046] The preparation method of the enzyme-based composite nanoprobe comprises the following steps:

[0047] S11: Will be used to prepare NaYF 4 : Er, Yb rare earth raw materials are mixed with oleic acid and 1-octadecene to prepare oleic acid-coated upconversion nanoparticles under nitrogen protection;

[0048] S12: Mix the prepared oleic acid-coated upconversion nanoparticles with the C18-PEG-CO...

Embodiment 2

[0052] This embodiment provides an enzyme-based composite nanoprobe and its preparation method. The structure of the enzyme-based composite nanoprobe is as follows figure 1 As shown, it is a core-shell structure, the particle size of the core body is 20nm, and the core body includes NaGdF 4, the doping elements are preferably Er and Yb; the shell material includes an oleic acid layer and a C18-PEG-COOH amphiphilic polymer combined with the oleic acid layer, and an oxygen-sensitive fluorescent molecule platinum octaethylene is combined in the shell porphyrin and D-amino acid oxidase.

[0053] The preparation method of the enzyme-based composite nanoprobe comprises the following steps:

[0054] S11: Will be used to prepare NaGdF 4 : Er, Yb rare earth raw materials are mixed with oleic acid and 1-octadecene to prepare oleic acid-coated upconversion nanoparticles under nitrogen protection;

[0055] S12: Mix the prepared oleic acid-coated upconversion nanoparticles with the C18...

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Abstract

The invention provides an enzyme-based composite nano-probe, its preparation method and application. The enzyme-based composite nanoprobe is a nano-core-shell structure, and its core body includes upconverting nanoparticles, and the material used to coat the shell layer of the core body includes an oleic acid layer for coating the core body, The amphiphilic polymer combined with the oleic acid layer is also loaded with oxygen-sensitive fluorescent molecules in the hydrophobic region jointly formed by the oleic acid layer and the amphiphilic polymer, and the amphiphilic polymer is also combined with D ‑Amino acid oxidase group. The enzyme-based composite nanoprobe effectively integrates the advantages of good detection effect of near-infrared light in vivo and high specificity of D-amino acid oxidase group through the synergistic effect between the contained core body and the shell layer, endowing it with High sensitivity, strong specificity, non-invasive and other advantages, so as to realize the efficient detection of D-amino acids in vivo. In addition, the enzyme-based composite nanoprobe has a stable structure and performance, and the process conditions of the preparation method are controllable, and the prepared enzyme-based composite nanoprobe has stable performance.

Description

technical field [0001] The invention relates to the technical field of nano-probe materials, in particular to an enzyme-based composite nano-probe and its preparation method and application. Background technique [0002] As we all know, amino acid is an important organic compound in the life system. For a long time, scientists have believed that only L-amino acid exists in higher organisms, and its enantiomer D-amino acid exists only in lower microorganisms such as bacteria. However, with the advancement of detection technology and the deepening of research, researchers have successively detected a variety of D-amino acids in the central nervous system, liver and gastric juice of mammals and humans. More meaningfully, more and more studies have shown that D-amino acid plays an important role in the development of various neurological diseases and the secretion of neurotransmitters, and it is also one of the markers of early gastric cancer. Therefore, it is of great signifi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00
CPCA61K49/0017A61K49/0056A61K49/0093
Inventor 王瑀唐永安刘小钢
Owner SHENZHEN UNIV
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