Intensified Daqu capable of producing tyrosol and hydroxytyrosol as well as preparation method and application
A technology of hydroxytyrosol and tyrosol, which is applied in the field of strengthening Daqu and its preparation, can solve the problems of breeding tyrosol and hydroxytyrosol-producing functional bacteria that have not been seen yet, and achieve improved taste comfort, good compatibility, and soft and sweet taste Effect
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Embodiment 1
[0021] A kind of preparation method of the strengthened Daqu capable of producing tyrosol and hydroxytyrosol, comprises the following steps:
[0022] (1) Prepare medium:
[0023] a Preparation of slant medium: Weigh 200g of potatoes, cut into small pieces, put in 1000ml of water, boil for 20-30 minutes, and filter the residue with a filter cloth. Add 2% glucose and 15-20 g of agar to the filtrate (crushed in advance), heat and melt, add water to make up to 1000 mL, the pH value is natural, divide into test tubes, and sterilize at 121 ° C for 30 min.
[0024] b Preparation of liquid seed medium: Weigh 200g of potatoes, cut into small pieces, put in 1000ml of water, boil for 20-30 minutes, and filter out the residue with a filter cloth. Add 2% glucose, 0.3% KH to the filtrate 2 PO 4 , 0.15% MgSO 4 ·7H 2 O. 0.15% yeast extract, adjust the pH to neutral with NaOH aqueous solution, distribute in Erlenmeyer flasks, and sterilize at 121°C for 30min.
[0025] c Preparation of so...
Embodiment 2
[0033] A kind of preparation method of the strengthened Daqu capable of producing tyrosol and hydroxytyrosol, comprises the following steps:
[0034] (1) Prepare medium:
[0035] Preparation of solid medium: Add 5.0% peptone aqueous solution according to 40% of the weight of rice or wheat, mix well, put in 500 ml Erlenmeyer flasks, 100 grams per bottle, and sterilize at 121° C. for 30 minutes.
[0036] (2) Screening strains: Take 10 g of Daqu sample and put it into a conical flask filled with 100 mL of sterile water, then add sterile glass beads, place on a shaker, and shake at 28°C for 30 minutes. After standing still, the bacterial solution was diluted to 10 times according to the 10-fold gradient. -6, take 1mL of each dilution, put it into a petri dish filled with enriched medium cooled to 50°C after melting, mix evenly, and put it in a constant temperature incubator at 30°C for 7 days after solidification. The grown colonies were picked and purified by streaking to obtai...
Embodiment 3
[0040] A kind of preparation method of the strengthened Daqu capable of producing tyrosol and hydroxytyrosol, comprises the following steps:
[0041] (1) Prepare medium:
[0042] Preparation of solid medium: add 2.5% peptone aqueous solution according to 40% of the weight of rice or wheat, mix well, pack in 500 ml Erlenmeyer flasks, 100 grams per bottle, and sterilize at 121° C. for 30 minutes.
[0043] (2) Screening strains: Take 10 g of Daqu sample and put it into a conical flask filled with 100 mL of sterile water, then add sterile glass beads, place on a shaker, and shake at 28°C for 30 minutes. After standing still, the bacterial solution was diluted to 10 times according to the 10-fold gradient. -6 , take 1mL of each dilution, put it into a petri dish filled with enriched medium cooled to 50°C after melting, mix evenly, and put it in a constant temperature incubator at 30°C for 6 days after solidification. The grown colonies were picked and purified by streaking to obt...
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