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Fusidic acid high-yield strain as well as breeding method and application thereof

A technology for fusidic acid and high-yielding strains, which is applied in the field of fusidic acid high-yielding strains and their breeding, can solve the problems of complicated chemical synthesis steps, difficult application of chemical methods, low yield and the like, and achieves easy implementation and cultivation conditions. Gentle, easy-to-use effect

Active Publication Date: 2019-03-15
YANCHENG INST OF TECH
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  • Abstract
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Problems solved by technology

Fusidic acid is a steroidal compound. Due to the existence of an asymmetric center in its molecule, the chemical synthesis steps are cumbersome, the cost is high, and the yield is low. It is difficult to apply chemical methods to actual industrial production. At present, microbial fermentation is mainly used at home and abroad. production method

Method used

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  • Fusidic acid high-yield strain as well as breeding method and application thereof
  • Fusidic acid high-yield strain as well as breeding method and application thereof
  • Fusidic acid high-yield strain as well as breeding method and application thereof

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Embodiment 1

[0032] (1) Preliminary screening of fusidic acid-producing strains resistant to strong polar organic solvents

[0033] The starting strain Fusidium coccineum (Fusidium coccineum, screened from the soil of Nanjing Laoshan Scenic Area) was inserted into the primary seed medium (glucose 2.5%, fish powder peptone 1.0%, yeast powder 0.2%, KH 2 PO 4 0.1%, MgSO 40.05%, corn steep liquor 4.0%, light calcium carbonate 0.2%, pH5.9±0.1), cultured at 27°C for 72±5h, then transferred to the secondary expansion medium (the same level of seed medium as the formula) ), cultured at 27°C for 25±5h, and then transferred to the fermentation medium (10.0% sucrose, 0.5% gluten powder, 2.0% yeast powder, 1.0% corn steep liquor, 0.5% soybean cake powder, K H 2 PO 4 0.1%, MgSO 4 0.05%, light calcium carbonate 0.3%, pH 5.9±0.1), culture at 27°C for 3 days, draw 1ml of bacterial suspension into a 2ml sterile centrifuge tube, add DMSO (dimethyl sulfoxide), the final concentration of DMSO is Volu...

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Abstract

The invention discloses a fusidic acid high-yield strain as well as a breeding method and application thereof. The name of the fusidic acid high-yield strain is fusidium coccineum NJWW-0520, preservedin the Center for General Microbiology of the China Microbial Culture Collection Management Committee on March 21, 2018 with the preservation number of CGMCC No.15473. The fusidic acid high-yield strain is bred by stress-oriented domestication of a fusidic acid producing strain by continuously using DMSO (dimethyl sulfoxide) for a long time. The yield of fusidic acid produced by fermentation of the domesticated strain is as high as 4,932 [mg]g / ml, which is 53.88 percent higher than that of the original strain (3,205 [mg]g / ml), and the fusidic acid high-yield strain is fully applied in efficient preparation of the fusidic acid by an actual biological method.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering and microbial strain breeding, and in particular relates to a high-yield fusidic acid strain, a breeding method and application thereof. Background technique [0002] Fusidic acid, the molecular formula is C 31 h 48 o 6 , molecular weight 516.69, chemical name: trans-16α-carboxy-3β, 11β-dihydroxy-4β, 8β, 14α-trimethyl-18-desmethyl-5β, 10α-cholesteryl-(17Z)-17 (20), 24-diene-21-acid hemihydrate, has a steroid-like structure, and its molecular structure has a high degree of similarity with fumagic acid and cephalosporins, and there are differences in a small number of groups. The presence of the group enhanced its antibacterial effect. Experiments have shown that fusidic acid has very little water solubility and good fat solubility, while sodium fusidate is easily soluble in water. [0003] Fusidic acid is an important narrow-spectrum antibiotic with + bacteria have strong inh...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/02C12P33/00C12R1/645
CPCC12N1/02C12N1/14C12P33/00C12N1/145C12R2001/645
Inventor 高健黄巍巍徐虹李军张丽薛锋倪浩熊璐璐
Owner YANCHENG INST OF TECH
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