Method for improving induction effect of agapanthus praecox ssp .orientalis somatic embryo seedlings

A technique for Agacia japonica and embryo seedlings is applied in horticultural methods, botanical equipment and methods, horticulture and other directions, which can solve the problems of a small number of mature embryos induced, a low number of somatic embryo seedlings, embryo abortion, etc., so as to reduce deformed seedlings. The occurrence of , the effect of improving the induction efficiency and morphological integrity

Active Publication Date: 2019-03-19
XINYANG AGRI & FORESTRY UNIV
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  • Claims
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Problems solved by technology

[0006] The purpose of the present invention is to solve the problem that the number of mature embryos induced by embryogenic cells of Agapanthus in the process of frequent subculture is s

Method used

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  • Method for improving induction effect of agapanthus praecox ssp .orientalis somatic embryo seedlings
  • Method for improving induction effect of agapanthus praecox ssp .orientalis somatic embryo seedlings
  • Method for improving induction effect of agapanthus praecox ssp .orientalis somatic embryo seedlings

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Embodiment 1

[0032] A method for improving the induction effect of agapanthus body embryos, comprising the steps of:

[0033] (1) Material collection of explants: during the flowering period in May to June, uncracked small flower buds of Agapanthus 4 to 5 years old were taken, and disinfected on an aseptic operating table (firstly treated with 75% (v / v) ethanol for 1 min) , followed by ddH 2 O rinsed 4 times, then disinfected with 2% sodium hypochlorite for 5min), and then treated with ddH 2 0 rinse 4 times, blot the moisture on the surface of the small flower buds with sterile filter paper, then cut the small pedicel explants, and cut the small pedicel explants into small sections of 0.7~1.0cm;

[0034] (2) Callus induction: Take 0.7-1.0 cm small pedicel explants and inoculate them in the callus induction medium in a flat state, and culture them in the dark at 25°C for 15 days, white translucent callus can be seen ( figure 1 It is an anatomical microscope observation figure after the s...

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Abstract

The invention relates to a method for improving the induction effect of agapanthus praecox ssp .orientalis somatic embryo seedlings. The method comprises the steps of selecting explants, induction ofcalluses, sub-culturing of the calluses, induction of embryogenic calluses, induction of mature embryos of embryogenic cells and induction of the somatic embryo seedlings. According to the characteristics of hormone anabolism and signal transduction embryogenically started and maintained by agapanthus praecox ssp .orientalis, the calluses are induced by using small peduncles as the explants; embryonic calluses are obtained through sub-culturing and induction of the calluses; the ratio of sugar to hormone is changed and the cell polarity is controlled in the somatic embryo induction period so as to induce a large number of somatic embryos with simultaneous development, and the hormone synthesis and signal are regulated by optimizing an induction culture medium scheme in the induced somaticembryo development process to obtain the large number of somatic embryo seedlings with complete form and excellent growth vigor. With the method for improving the induction effect of the agapanthus africanus somatic embryo seedlings, the problems of a small number of induced mature embryos, embryo abortion and a small number of somatic embryo seedlings generated in the frequent subculturing process of the agapanthus africanus embryogenic cells are solved.

Description

technical field [0001] The invention belongs to the technical field of rapid multiplication of improved plant varieties, and in particular relates to a method for improving the induction effect of agapanthus body embryo seedlings. Background technique [0002] Agapanthus praecox ssp.orientalis, also known as "Blue Lily" and "African Lily", is native to South Africa and the Kingdom of Lesotho in southern Africa. It is a perennial herbaceous monocot flower with strong ornamental value. For nearly half a century, Agapanthus has stood out in the development of the international flower industry and has become a popular fresh-cut flower, potted plant and ground cover flower all over the world, and it has shown excellent ornamental value. Agapanthus is heat-resistant, drought-resistant, and has strong cold resistance. It has huge development space in the fields of road greening and soil restoration. At present, the supply of seedlings in the market is in short supply. [0003] Aga...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 岳建华董艳张琰李蒙夏维丽施芹川王苗
Owner XINYANG AGRI & FORESTRY UNIV
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