Method for populus diversifolia tissue culture seedling ex vitro rooting

Abstract

The invention relates to a method for populus diversifolia tissue culture seedling ex vitro rooting, comprising the following steps: moving a culture bottle of diversifolia tissue culture seedlings with seedling height growing to 6-8cm out of a culture room, and sealing the bottle for seedling hardening for 7-10 days; then opening the bottle for seedling hardening for 5-7 days; performing root induction pretreatment on the diversifolia tissue culture seedlings with a liquid culture medium, then transplanting into a seedling growing porous tray containing a rooting matrix, then thoroughly watering with root setting water, covering the seedling growing porous tray with a plastic film or a transparent container for moisturizing; spraying mist for two times with improved 1 / 2MS liquid culture medium each day in the earlier stage, wherein the culture room temperature is 25-30 DEG C, illumination intensity is 2000-2500lux, and after indoor culture of 20-30 days, rooting of the populus diversifolia tissue culture seedling is as long as 5-7cm; and finally performing field transplanting. The method has the advantages that the tissue culture seedling ex vitro rooting technology reduces the links of preparation, sterilization, inoculation and the like of the rooting culture medium, simplifies the production procedure, shortens the seedling growing time, and can enable the total seedling growing time to be shortened by more than 20 days; the final rooting rate is 99.3%, and the survival rate is 98%.

Description

technical field [0001] The invention relates to a method for rooting out of a bottle of Populus euphratica tissue culture seedlings. Background technique [0002] Populus euphratic Oliver (Populus euphratic Oliver) is a deciduous tree belonging to the phylum Angiosperms, the class Dicotyledons, the subclass Salicida, the order Salicidae, the family Salicaceae, and the genus Populus. Populus euphratica is different from ordinary poplars. It is mainly distributed in the desert areas of Northwest my country. It has tenacious vitality, can endure the arid climate in the desert, can take root to the ground above 20 meters to absorb water, and has strong tolerance to salinity and alkali. It is resistant to extreme temperature and wind and sand burial, and can adapt to extreme drought, changeable and harsh continental climate for a long time. It is the only tall tree species naturally distributed in the frontier of arid desert wind and sand. Populus euphratica forest plays an impor...

AI Technical Summary

Problems solved by technology

Although the tissue culture techniques of Populus euphratica, Populus euphratica, Populus tomentosa, and Populus alba have been relatively mature, the tissue culture system of other poplars cannot be applied at all due to the great differences in the physiological characteristics of Populus euphratica with other poplars.

At present, the tissue culture technology of Populus euphratica is not perfect, and there are still problems in the process of tissue culture of Populus euphratica, such as complicated culture process, long culture period, yellowing of tissue culture seedlings after multiple subcultures, difficulty in rooting of tissue culture seedlings, and low survival rate of transplanting And other issues