A kind of method of populus euphratica tissue culture seedling rooting outside the bottle

Abstract

The invention relates to a method for rooting out of a bottle of Populus euphratica tissue culture seedlings. The steps are as follows: remove the culture bottle of Populus euphratica tissue culture seedlings grown to a seedling height of 6-8 cm out of the cultivation room, close the bottle and train the seedlings for 7-10 days; open the bottle and practice the seedlings for 5-7 days; After being pre-treated by liquid medium to induce rooting, it was transplanted into a seedling tray filled with a rooting substrate. / 2MS liquid medium was sprayed twice, the temperature of the culture room was 25-30°C, and the light intensity was 2000-2500 lux. After 20-30 days of indoor cultivation, the Populus euphratica tissue-cultured seedlings took root up to 5-7 cm long; finally, they were transplanted in the field. Advantages of the present invention: the rooting technology of tissue cultured seedlings outside the bottle reduces the preparation of rooting medium, sterilization, inoculation and other links, simplifies the production procedure, shortens the time for seedling cultivation, and can shorten the total seedling cultivation time by more than 20 days; the final rooting rate is 99.3%, the survival rate was 98%.

Description

technical field [0001] The invention relates to a method for rooting out of a bottle of Populus euphratica tissue culture seedlings. Background technique [0002] Populus euphratic Oliver (Populus euphratic Oliver) is a deciduous tree belonging to the phylum Angiosperms, the class Dicotyledons, the subclass Salicida, the order Salicidae, the family Salicaceae, and the genus Populus. Populus euphratica is different from ordinary poplars. It is mainly distributed in the desert areas of Northwest my country. It has tenacious vitality, can endure the arid climate in the desert, can take root to the ground above 20 meters to absorb water, and has strong tolerance to salinity and alkali. It is resistant to extreme temperature and wind and sand burial, and can adapt to extreme drought, changeable and harsh continental climate for a long time. It is the only tall tree species naturally distributed in the frontier of arid desert wind and sand. Populus euphratica forest plays an impor...

AI Technical Summary

Problems solved by technology

Although the tissue culture techniques of Populus euphratica, Populus euphratica, Populus tomentosa, and Populus alba have been relatively mature, the tissue culture system of other poplars cannot be applied at all due to the great differences in the physiological characteristics of Populus euphratica with other poplars.

At present, the tissue culture technology of Populus euphratica is not perfect, and there are still problems in the process of tissue culture of Populus euphratica, such as complicated culture process, long culture period, yellowing of tissue culture seedlings after multiple subcultures, difficulty in rooting of tissue culture seedlings, and low survival rate of transplanting And other issues