The ribosomal RNA gene and application of erysiphe alphitoides pathogenic tussah powdery mildew

A powdery mildew and pathogenic bacteria technology, applied in the direction of DNA/RNA fragments, application, genetic engineering, etc., can solve the problems of uncertain pathogenic fungal species, high variability of ITS segments, fungal species research, etc., and achieve good application prospects Effect
CN109486836BActive Publication Date: 2021-02-19SOUTH CHINA AGRI UNIV

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SOUTH CHINA AGRI UNIV
Publication Date
2021-02-19

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Abstract

The invention discloses an oak powdery mildew pathogen Erysiphe alphitoides Ribosomal RNA genes and their applications. Oak powdery mildew pathogen Erysiphe alphitoides The nucleotide sequence of the ribosomal RNA gene is shown in SEQ ID NO.1, utilizes this sequence to be able to the oak tree powdery mildew pathogen Erysiphe alphitoides For detection, the sequence can also be applied to the study of fungal species classification. In addition, the present invention also provides a group of pathogenic bacteria for detecting oak powdery mildew Erysiphe alphitoides The primers have strong specificity, and based on the primers, a specific detection method for the pathogenic bacteria of oak powdery mildew was established. Erysiphe alphitoides The detection method and detection kit, in the detection of oak powdery mildew pathogen Erysiphe alphitoides has a good application prospect.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and more specifically relates to an oak powdery mildew pathogen Erysiphealphitoides ribosomal RNA gene and its application. Background technique

[0002] In the existing fungal research methods, ribosomal DNA (ribosome DNA, rDNA) sequences are often sequenced and compared for the identification of fungi. Ribosomes have important functions in cells, and many genes encoded by rDNA are closely related to the reaction process of protein synthesis and play a decisive role in protein biosynthesis. The rDNA sequence is divided into a transcribed region and a non-transcribed region. The transcribed region consists of genes encoding ribosomal 5.8S, 18S, and 28S protein structures and two intergenic transcribed spacers (Internal Tanscribed Spacer, ITS) ITS1 and ITS2. form a transcription unit.

[0003] The rDNA sequences encoding 5.8S, 18S, and 28S in rDNA are relatively conserved and can be used to analyze ...

Claims

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