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A kind of coconut immature inflorescence tissue culture method

A tissue culture, immature technology, applied in the field of coconut immature inflorescence tissue culture, can solve problems such as poor test repeatability, no success, long time, etc., and achieves a method of improving callus induction rate, shortening tissue culture period, and stable method. Effect

Active Publication Date: 2021-08-10
COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Coconut is a highly heterozygous plant, and none of the culture media and cultivation methods published so far is widely applicable, and they are all limited to a certain type of genotype, or even in the same variety, some Plants can successfully induce callus, while some cannot
The Coconut Research Institute in Sri Lanka has a specialized tissue culture center, and they have been facing this problem for decades, which is difficult to overcome
[0006] 2. Poor test repeatability
The regenerated plants obtained by the Philippine Coconut Agency for the first time have already set fruit, but subsequent experiments have not been successful
[0008] 3. The tissue culture cycle is long
It will take longer to establish a mature technology system that can be used on a large scale
[0010] In addition, problems such as low reproductive coefficient and difficult reproduction need to be solved urgently.
[0011] In summary, there is currently no mature technical solution available for production

Method used

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  • A kind of coconut immature inflorescence tissue culture method
  • A kind of coconut immature inflorescence tissue culture method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] A coconut immature inflorescence tissue culture method, including the following steps:

[0060] The immature inflorescence of the cultivation of the coconut No. 2 is used as an explant; in turn, through the callus and induction stage, the invocation stage, the body embryo induction stage, the body embryo maturity stage, the regeneration plant structure stage and group The cultivation stage of the cultivation culture stage of the seedlings;

[0061] 1.1 Callus induction stage:

[0062] The callus induced medium includes the following ingredients: 250 μm 2, 4-D, 5 μm BAP, 5 μm 2 IP, 4G / L plant gel, 3 g / L activated carbon and 30 g / L sucrose were added to the modified Y3 medium.

[0063] The pH of the medium was 5.75, and the culture conditions were in dark culture, and the culture time was 1 month.

[0064] 1.2 Callus and expansion phase:

[0065] Callus and spread developments include the following ingredients: 250 μm 2, 4-D, 5 μm BAP, 5 μM 2 IP, 10μm BAP, 4G / L plant ...

Embodiment 2

[0081] A coconut immature inflorescence tissue culture method, including the following steps:

[0082] The immature inflorescence of the cultivation of the coconut No. 2 is used as an explant; in turn, through the callus and induction stage, the invocation stage, the body embryo induction stage, the body embryo maturity stage, the regeneration plant structure stage and group The cultivation stage of the cultivation culture stage of the seedlings;

[0083] 1.1 Callus induction stage:

[0084] The callus-induced medium includes the following ingredients: 200 μm 2, 4-D, 3 μm BAP, 3 μm 2 IP, 2G / L plant gel, 1 g / L activated carbon and 20 g / L sucrose were added to the modified Y3 medium;

[0085] The pH of the medium was 5.75 and the culture conditions were muddling culture, and the culture time was 1 month.

[0086] 1.2 Callus and expansion phase:

[0087] The callus developing medium includes the following ingredients: 200 μm 2, 4-D, 3 μm BAP, 3 μm 2 IP, 5μm BAP, 2G / L plant ge...

Embodiment 3

[0102] A coconut immature inflorescence tissue culture method, including the following steps:

[0103] The immature inflorescence of the cultivation of the coconut No. 2 is used as an explant; in turn, through the callus and induction stage, the invocation stage, the body embryo induction stage, the body embryo maturity stage, the regeneration plant structure stage and group The cultivation stage of the cultivation culture stage of the seedlings;

[0104] 1.1 Callus induction stage

[0105] The callus induced medium includes the following ingredients: 300 μm 2, 4-D, 8 μm BAP, 8 μm 2 IP, 8G / L plant gel, 8 g / L activated carbon and 40 g / L sucrose were added to the modified Y3 medium.

[0106] The pH of the medium was 5.80, the culture conditions were mad culture, and the culture time was 3 months.

[0107] 1.2 Callus and expansion phase

[0108] The callus developing medium includes the following ingredients: 300 μm 2, 4-D, 8 μm BAP, 8 μm 2 IP, 20μm, 8 g / L, 8 g / L activated ...

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Abstract

The invention discloses a tissue culture method of coconut immature inflorescences. Using coconut immature inflorescences as explants, the medium formula is strictly controlled at each stage of tissue culture. After tissue culture, the highest callus induction rate can reach 97.2%. The highest embryo induction rate can reach 98.0%, the highest somatic embryo regeneration rate can reach 99.1%, and the highest transplant survival rate can reach 97.5%. The medium formula and method of the present invention are more broad-spectrum, suitable for many varieties such as Hainan local high species, Wenye No. 2, Wenye No. 3, etc., and have good repeatability and short cycle, which is conducive to industrialization and commercialization. .

Description

Technical field [0001] The present invention relates to a coconut immature inflorescence tissue culture method, belonging to the field of plant tissue culture technology. Background technique [0002] Tissue culture technology is a method of rapid reproductiveness in exhibitors such as roots such as plants, stems, leaves, flowers, and fruits, have been successfully obtained on various plants. Tissue culture technology is the most effective amorphous breeding method of coconut rapid reproduction. As early as 1978, Fisher and Tsai tried to tissue regeneration with coconut embryos, but only won the callus, no recycled plants. In 1994, Verdeil et al. First successfully obtained regenerative plants from coconut immature. Although thereafter, the test tube seedlings produced by various research groups were still unreliable and repeated. Chan et al. (1998) obtained regenerated plants from coconut germs through body embryo. Fernando et al. (2000) Using coconut for 7-9 months immature ger...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 范海阔刘蕊
Owner COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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