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Unicellular microcystis flocculation method

A microcystis and single-cell technology, applied in the field of water treatment, can solve the problems of increased treatment costs, environmental risks, and increased sludge volume, and achieve the effects of reduced fluorescence, effective flocculation and inactivation, and cell integrity

Inactive Publication Date: 2019-03-26
HOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these added chemical flocculants not only require complex follow-up processes to remove (increase treatment costs and increase sludge volume), but also have environmental risks in their residues
[0004] Currently there is no safe, simple and effective way to remove Microcystis

Method used

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  • Unicellular microcystis flocculation method
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  • Unicellular microcystis flocculation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Culture of Microcystis aeruginosa: Microcystis aeruginosa species (FACHB-469) was purchased from the Freshwater Algae Species Bank of the Chinese Academy of Sciences, and cultured in a light incubator with BG11 medium at a temperature of 25 °C and a light condition of 30 μmol m -2 the s -1 , The light-to-dark ratio is 12h:12h. Algae cells were cultured to the logarithmic phase before the experiment.

[0025] pH adjustment, particle size measurement of algal cell flocculation: take algal cells and inoculate them into fresh BG-11 medium, and prepare a cell concentration of 10 7 For cells / mL of algae suspension, slowly add 1mol / L dilute hydrochloric acid into the prepared algae suspension, stir well, and adjust the pH of the algae suspension to 4, 3, and 2 respectively. At the same time, 0.25mol / L NaOH was used to adjust the pH of the algae suspension to 12. The pH of the blank control group was 11. After the algal suspension with adjusted pH was left to stand for 30 m...

Embodiment 2

[0028] Culture of Microcystis aeruginosa: Microcystis aeruginosa species (FACHB-469) was purchased from the Freshwater Algae Species Bank of the Chinese Academy of Sciences, and cultured in a light incubator with BG11 medium at a temperature of 25 °C and a light condition of 30 μmol m -2 the s -1 , The light-to-dark ratio is 12h:12h. Algae cells were cultured to the logarithmic phase before the experiment.

[0029] pH adjustment and algal cell activity determination: take algal cells and inoculate them into fresh BG-11 medium, and prepare a cell concentration of 10 7 cells / mL algae suspension, divided into 9 groups: slowly add 1mol / L dilute hydrochloric acid into the prepared algae suspension, stir well, until the pH of the algae suspension is 4, 3, 2.9, 2.8, 2.7, 2.6 , 2.0; while using 0.25mol / L NaOH to adjust the pH of the algae suspension to 12; the pH of the blank control group = 10. After the algal suspension with adjusted pH was allowed to stand for 30 min, the activi...

Embodiment 3

[0032] Culture of Microcystis aeruginosa: Microcystis aeruginosa species (FACHB-469) was purchased from the Freshwater Algae Species Bank of the Chinese Academy of Sciences, and cultured in a light incubator with BG11 medium at a temperature of 25 °C and a light condition of 30 μmol m -2 the s -1 , The light-to-dark ratio is 12h:12h. Algae cells were cultured to the logarithmic phase before the experiment.

[0033] pH adjustment, release of algal cell contents: take algal cells and insert them into fresh BG-11 medium, and prepare cells at a concentration of 10 7 The cells / mL algae suspension is divided into 5 groups: Use distilled water to make concentrated hydrochloric acid into 1mol / L dilute hydrochloric acid, then slowly add 1mol / L dilute hydrochloric acid into the prepared algae suspension, and fully stir to adjust the algae suspension The pHs were 4, 3, and 2 respectively; the pH of the blank control group was 11. The algae suspension with adjusted pH was left to stand...

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Abstract

The invention provides a unicellular microcystis flocculation method, and relates to the technical field of water treatment. The method comprises the following steps that the pH value of unicellular microcystis suspension is regulated to be 2.0 to 4.0; still standing is performed for 25 to 40min; cell precipitates of the unicellular microcystis are removed. According to the method, when the pH isregulated to 2 to 4, aggregates can be effectively formed; precipitation or filtering separation is easy; in the pH range, the microcystis fluorescence is obviously reduced; the cell is inactivated; meanwhile, the cell is complete; intracellular protein is not leaked. When the pH is regulated to be in a range of 2 to 2.7, the water quality pollution due to microcystis cell rupture can be avoided;the effective flocculation and inactivation are realized.

Description

technical field [0001] The invention belongs to the technical field of water treatment, and in particular relates to a flocculation method for single-cell Microcystis. Background technique [0002] Global warming and the continuous increase of nitrogen and phosphorus nutrients in water bodies have exacerbated the outbreak of Microcystis blooms (common cyanobacterial blooms). Some species of Microcystis can release microcystin, which is harmful to aquatic animals, plants and humans. The outbreak of Microcystis blooms can lead to the release of a large amount of microcystins, black and smelly water, and trigger a water supply crisis. [0003] Microcystis mainly in the form of groups in the wild. However, recent studies have shown that there are still a large number of unicellular Microcystis in some lakes (such as: Taihu Lake) (Zhu et al. 10 5 cells / mL). Compared with the group form, the unicellular Microcystis is large and scattered, difficult to separate, difficult to c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F9/04C02F1/66
CPCC02F1/66C02F2001/007
Inventor 谭啸
Owner HOHAI UNIV