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Online concentration determination method for disodium 5'-inosinate and disodium 5'-guanylate

A technology of disodium guanylate and disodium inosinate, which is applied in the direction of color/spectral characteristic measurement, etc., can solve problems such as the absence of online concentration detection methods, and achieve the effect of avoiding influence

Active Publication Date: 2019-03-26
肇东星湖生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The present invention is to solve the problem of lack of online concentration detection method in the crystallization process of the dual-solute ethanol water system of IMP and GMP. IMP and GMP dual-solute ethanol water system in-situ online real-time concentration detection method and supporting device for solvent crystallization, evaporation crystallization and other processes

Method used

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  • Online concentration determination method for disodium 5'-inosinate and disodium 5'-guanylate
  • Online concentration determination method for disodium 5'-inosinate and disodium 5'-guanylate
  • Online concentration determination method for disodium 5'-inosinate and disodium 5'-guanylate

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Experimental program
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Effect test

Embodiment 1

[0086] The solubility of IMP and GMP in the mixed solvent of ethanol and water with a mass ratio of 0 to 40% is measured online at different temperatures. IMP and GMP solubility measurement is to determine the concentration of IMP and GMP in a saturated solution of IMP and GMP.

[0087] Before the on-line concentration measurement of IMP and GMP, it is necessary to obtain the correlation coefficient of the on-line detection model. The specific steps of the correlation coefficient regression process of the detection model are as follows:

[0088] 1) Obtain calibration data.

[0089] In this embodiment, according to the needs of the IMP and GMP solubility measurement process, the variation range of the ethanol concentration is 0-0.4g / g water, the variation range of the solution temperature is taken as 5-75°C, and the variation range of the IMP and GMP component concentration levels The lower limit is 0, and the upper limit is the saturated solubility at 75°C in a pure water en...

Embodiment 2

[0131] IMP and GMP cooling crystallization process online concentration detection, the solvent is 20% ethanol water.

[0132] In this embodiment, according to the needs of IMP and GMP cooling crystallization process, the ethanol concentration is fixed at 0.2g / g water, the variation range of solution temperature is 20-70°C, and the variation range of IMP and GMP component concentration levels is 0-0.3 g / g solvent.

[0133] In this embodiment, the temperature-lowering crystallization operation only changes the solution temperature, and the solvent composition does not change. Therefore, the concentration calibration matrix of PLS ​​method modeling regression should fix the proportion of ethanol, only change the concentration of IMP and GMP and the solution temperature, avoid introducing too much noise to reduce the modeling accuracy, and reduce the workload of concentration calibration. For the purpose of convenience, this embodiment directly follows the online concentration de...

Embodiment 3

[0141] IMP and GMP ethanol anti-solvent crystallization process online concentration detection, ethanol as anti-solvent.

[0142] In this embodiment, according to the needs of the IMP and GMP ethanol anti-solvent crystallization process, the solution temperature is fixed at 60°C, the concentration variation range of the anti-solvent ethanol is taken as 0-0.4g / g water, and the concentration level variation range of IMP and GMP components Take 0~0.2g / g solvent.

[0143] In this embodiment, the ethanol anti-solvent crystallization operation only changes the concentration of anti-solvent ethanol, and the solution temperature does not change. Therefore, the concentration calibration matrix of PLS ​​method modeling regression should fix the solution temperature and ethanol ratio, only change the concentration of IMP and GMP and the concentration of anti-solvent ethanol, avoid introducing too much noise to reduce the modeling accuracy, and reduce the workload of concentration calibra...

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Abstract

The invention discloses online concentration determination method for disodium 5'-inosinate and disodium 5'-guanylate. According to the method, calibration data is firstly acquired, regression modelsare determined, and online concentration determination is carried out. The regression models are formula and formula (shown in the description), wherein CIMP and CGMP are respectively the concentrations of IMP and GMP solutions, Ri is the absorbance value of an ATR-UV absorption spectrum in a wavelength range of 230nm-310nm, cEtOH is the ethanol concentration, T is the solution temperature, and alphai,IMP, betaIMP, gammaIMP, epsilonIMP, alphai,GMP, betaGMP, gammaGMP and epsilonGMP are correlation coefficients. According to the method, the online concentration detection of IMP and GMP double-solute systems is realized; and the method has the advantages has the advantages of large application range, simplicity in operation, instantaneity and the like and is suitable for the processes of crystallization and the like of IMP and GMP.

Description

technical field [0001] The invention relates to an online concentration detection method, and in particular to an online concentration detection device and method for disodium 5'-inosinate and disodium 5'-guanylate, belonging to the technical field of online detection. Background technique [0002] Disodium 5'‐inosinate (IMP for short) and disodium 5'‐guanylate (GMP for short) are common nucleotide compounds widely used in food , medicine and other fields. It is an important food additive in the food field. It has a strong effect of increasing freshness and inhibiting odor. It has a strong synergistic effect when used in combination with glutamic acid, which can increase the effect of increasing freshness by 10 to 20 times. In addition, IMP has a certain auxiliary effect on leukopenia and thrombocytopenia, as well as various acute and chronic liver diseases. The two are often mixed according to the mass ratio of 1:1, which is called 5'‐flavored nucleotide disodium (Disodiu...

Claims

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Application Information

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IPC IPC(8): G01N21/33
CPCG01N21/33
Inventor 江燕斌李炳辉宋阳孙良杰
Owner 肇东星湖生物科技有限公司