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A kind of detection method of amylase

A detection method, the technology of amylase, applied in the field of inspection, can solve the problems of complex operation and cumbersome calculation process, and achieve the effect of simple sample processing, high detection accuracy and small sample volume

Active Publication Date: 2022-07-05
JUNLEBAO DAIRY GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The above method is complicated to operate and the calculation process is cumbersome. The present invention aims to provide a fast, simple and intuitive detection method for amylase

Method used

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  • A kind of detection method of amylase
  • A kind of detection method of amylase
  • A kind of detection method of amylase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1 A kind of detection method of amylase in white granulated sugar

[0040] (1) Mix 40g white granulated sugar, 40g pure water and 20g starch paste with pH 6.6, heat to 95°C in a water bath while stirring, and keep the temperature for 5 minutes to obtain the experimental solution;

[0041] (2) The experimental solution prepared in step (1) was allowed to stand in a water bath at 75°C for 24 hours to obtain the solution to be tested;

[0042] (3) Shake the solution to be tested prepared in step (2), take 5 μL of the solution to be tested and 10 μL of potassium iodide-iodine solution on a glass slide, spread evenly with a glass rod, cover with a cover glass, and observe under a microscope Morphology of starch granules.

Embodiment 2~6

[0043] The detection method of amylase in embodiment 2~6 white granulated sugar

[0044] Embodiments 2 to 6 are respectively a method for detecting amylase in white granulated sugar, and the operation steps are the same as those in Example 1, except that the control parameters such as the addition amount of raw materials, reaction temperature, reaction time and sampling amount are different. See the table below:

[0045]

Embodiment 7

[0046] Embodiment 7 A kind of detection method of amylase in glucose

[0047] (1) Mix 40g of glucose, 40g of pure water and 20g of starch paste with pH 6.8, heat to 95°C in a water bath while stirring, and keep the temperature for 5 minutes to obtain the experimental solution;

[0048] (2) The experimental solution prepared in step (1) was allowed to stand in a water bath at 75°C for 24 hours to obtain the solution to be tested;

[0049] (3) Shake the solution to be tested prepared in step (2), take 5 μL of the solution to be tested and 10 μL of potassium iodide-iodine solution on a glass slide, spread evenly with a glass rod, cover with a cover glass, and observe under a microscope Morphology of starch granules.

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Abstract

The invention discloses a detection method for amylase. The principle of the detection method is to mix a sample to be tested and starch, dissolve it in water, dye it with potassium iodide-iodine solution, observe the shape of starch granules under a microscope, and observe the shape of starch granules under a microscope. The morphological change judges the presence or absence of amylase in the sample to be tested. If the starch granules swell obviously, the color becomes lighter or the granules disappear, it means that the sample to be tested contains amylase. If there is no change in the morphology of starch granules, it means that there is no amylase in the sample to be tested. The detection method provided by the invention is easy to operate, the results are intuitive and easy to understand, the detection precision is high, the sample processing is simple, and the required sample amount is small.

Description

technical field [0001] The invention belongs to the technical field of inspection, in particular to a method for detecting amylase. Background technique [0002] The full name of amylase is 1,4-α-D-glucanohydrolase, which catalyzes the hydrolysis of starch and glycogen to generate glucose, maltose and dextrin containing branched chains of α-1,6-glycosidic bonds. The raw and auxiliary materials for preparing yogurt contain many kinds of sugars, such as sugar, glucose, whey protein powder, fruit jam and so on. If the raw and auxiliary materials contain amylase, it will have an important impact on the quality of yogurt. Therefore, the detection of amylase in yogurt raw and auxiliary materials is of great significance. [0003] At present, there are 4 types of methods for the determination of amylase: [0004] One is to measure the consumption of substrate starch, including viscosity method, turbidity method and iodine-starch colorimetric method, etc.; the second is to genera...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/40
CPCC12Q1/40
Inventor 李建磊刘凤昝王少波张广辉岳旭良杨新尧苏海涛于文花姚欢付翠霞刘豆康志远王红叶
Owner JUNLEBAO DAIRY GRP CO LTD