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A method for breeding Wuding chickens based on parathyroid hormone blood biochemical markers

A parathyroid hormone, Wuding chicken technology, used in biological testing, material inspection products, color/spectral property measurement, etc.

Active Publication Date: 2021-09-24
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no research on the effect of parathyroid hormone on the bone development of Wuding chickens. In order to meet people's needs for local chicken breeds, it is necessary to propose a new method for breeding Yunnan Wuding chickens with good bone development. Research on hormones and bone development, establish a blood biochemical marker, pave the way for early breeding of Yunnan Wuding chicken

Method used

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  • A method for breeding Wuding chickens based on parathyroid hormone blood biochemical markers
  • A method for breeding Wuding chickens based on parathyroid hormone blood biochemical markers
  • A method for breeding Wuding chickens based on parathyroid hormone blood biochemical markers

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Collection and storage of experimental samples

[0022] (1) Preparation of serum

[0023] 20 Wuding chickens with normal skeletal development and abnormal skeletal development were randomly selected from the Wuding chicken family with normal skeletal development and Wuding chicken with abnormal skeletal development. Metabolism-related biochemical indicators. Water and food were cut off for 12 hours before blood collection, and blood was collected from the wing vein the next morning. Collect 3-4ml of blood sample in a 10ml centrifuge tube, let it stand for 30min, centrifuge at 3000rpm at room temperature for 20min, take the supernatant (serum) into a 1.5ml centrifuge tube, and store it in a -20°C refrigerator for later use.

[0024] (2) Collection of leg bone samples

[0025] 20 Wuding chickens with normal skeletal development and abnormal skeletal development were slaughtered at 1 and 2 months respectively, and the femurs and tibias of the left and right legs of t...

Embodiment 2

[0063] 100 one-month-old Wuding chickens with consistent body size and good health were selected in Wuding, Yunnan and its farms, and the PTH concentration in their blood was detected respectively. According to the obtained concentration, statistical grouping was carried out within a certain range, and the following statistical table was obtained. .

[0064] Table 9 PTH content statistics of one-month-old Wuding chickens

[0065]

[0066] These 5 groups of one-month-old Wuding chickens were raised to two-month-old under the same feeding conditions in strict accordance with the immunization program, and their tibial bone mineral density was measured respectively (using a portable bone densitometer to detect). The statistical results of each group are as follows (see table 10).

[0067] Table 10 Statistical statistics of average tibial bone density of one-month-old Wuding chickens

[0068]

[0069] It can be seen from Table 10 that the average tibial bone mineral density...

Embodiment 3

[0081] 1. Preparation of experimental materials

[0082] A total of 120 one-month-old Wuding chickens (including normal and abnormal bone development types) and 120 two-month-old Wuding chickens (including normal and abnormal bone development types) were sampled in Wuding chicken farms in Wuding County, Yunnan Province. Skeletal dysplasia type), measure the bone density of the tibia respectively; take blood from the wing vein, collect the blood sample with a 5ml centrifuge tube, let it stand for 30 minutes, and centrifuge at 3000rpm for 20 minutes at room temperature, and take the supernatant, which is extremely serum.

[0083] 2. Detection of PTH concentration in serum

[0084] Carry out strict specification operation according to PTH enzyme-linked immunoassay (ELISA) kit, apply double antibody sandwich method to measure the level of chicken osteocalcin (PTH) in the sample, measure absorbance (OD value) at 450nm wavelength with microplate reader, pass The standard curve was ...

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Abstract

The invention relates to a method for breeding Wuding chickens based on parathyroid hormone blood biochemical markers, which belongs to the technical field of poultry blood biochemical markers. The invention specifically includes the following steps: 1) collecting blood samples of Wuding chickens to be tested, preparing serum, Detect the concentration value of its parathyroid hormone (PTH); 2) compare the concentration value measured in step 1) with the blood biochemical markers of Wuding chickens, and distinguish the Wuding chickens to be tested into Wuding chickens with normal bone development and Wuding chickens Wuding chicken with abnormal skeletal development. In the present invention, by measuring the concentration change of parathyroid hormone (PTH) in Wuding chickens with normal skeletal development and abnormal skeletal development, combined with the detected bone density and bone strength of Wuding chickens, the normal and abnormal skeletal development types are given. The blood biochemical markers of Wuding chickens with abnormal skeletal development can screen high-quality chickens, provide a theoretical basis for the early breeding of Wuding chickens, and also explore the breeding methods of Wuding chickens, and provide new ideas for the breeding of Yunnan local chicken breeds. train of thought.

Description

technical field [0001] The invention belongs to the technical field of poultry blood biochemical markers, and in particular relates to a method for breeding Wuding chickens based on parathyroid hormone blood biochemical markers. Background technique [0002] As an important organ in the poultry, the bone functions to support, protect and exercise the poultry's body. In the past century, the high-intensity production performance of poultry has had a negative impact on poultry bone development, resulting in increased bone development defects, osteoporosis, rickets and other bone diseases, affecting the healthy development of the poultry industry. Chicken bone density and strength are important economic traits of chickens. Every year, bone diseases such as bone development defects, osteoporosis, and rickets cause huge economic losses to the chicken industry. Studies in the United States have shown that the mortality rate caused by skeletal deformities is about 1.5%-3.2%, indic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/78G01N33/53G01N21/31
CPCG01N21/31G01N33/53G01N33/78
Inventor 闫世雄贾俊静豆腾飞李涛葛长荣徐志强段小花谷大海刘丽仙黄英李琦华荣华张霞王坤孙帅程志斌
Owner YUNNAN AGRICULTURAL UNIVERSITY