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Cultivation method for beautiful millettia roots

A breeding method, Niu Dali's technology, which is applied in the field of traditional Chinese medicine Niu Dali's cultivation, can solve the problems of difficult disposal of waste medium, high sugar content in the medium, and complex fungicide components, etc., and achieve easy plant absorption and utilization, and transplant survival The effect of high yield and shortened incubation time

Inactive Publication Date: 2019-04-02
GUANGXI MEDICAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Patent CN201810094607-a planting method for improving the yield of Niu Dali discloses the use of Niu Dali stems as explants to cultivate Niu Dali seedlings; the induction medium is MS medium with 30g / L of sucrose, 6g / L of agar and 0.3mg of zeatin / L; Proliferation medium is MS medium with sucrose 50g / L, agar 10g / L, naphthaleneacetic acid 0.2mg / L and gibberellin 0.2mg / L; Rooting medium is MS medium with sucrose 60g / L, agar 15g / L, sodium dihydrogen phosphate 6g / L, ammonium nitrate 8g / L, naphthaleneacetic acid 0.5mg / L and zeatin 0.6mg / L; the patent has complex fungicide components and high sugar content in the medium during tissue culture , the waste medium is difficult to handle
Patent CN201710767249 - A method for cultivating Niu Dali discloses that Niu Dali's seeds are used for tissue culture to cultivate Niu Dali seedlings, but the price of Niu Dali's seeds is relatively high, resulting in higher cultivation costs

Method used

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  • Cultivation method for beautiful millettia roots
  • Cultivation method for beautiful millettia roots
  • Cultivation method for beautiful millettia roots

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Embodiment 1

[0024] A method for cultivating cattle vigorously comprises the following steps:

[0025] (1) Disinfection of explants: Select the twigs of the semi-lignified stems of Niu Dali, rinse them with clean water first, then cut them into small stems with a length of 0.5-1.0 cm, dry the surface water with filter paper, and dry them in a 3% filter paper. Sterilize in hydrogen peroxide solution for 60 seconds, wash twice with sterile water, blot the surface moisture with sterile paper, immerse in 0.1% mercuric acid solution for sterilization for 12 minutes, wash with sterile water for 5 times, use sterile paper Blot surface moisture.

[0026] (2) Induction cultivation: transfer the sterilized explants into the induction medium to induce differentiation, the cultivation conditions: the temperature is 23-27°C, the light time is 12 h / day, and the light intensity is 1600-2000lx; the induction medium is : MS + sucrose 20g / L + carrageenan 4g / L + 6-BA 2mg / L +KT 1mg / L +NAA 0.4mg / L +AC 1g / L, t...

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Abstract

The invention discloses a cultivation method for beautiful millettia roots. The cultivation method comprises the following steps: selecting beautiful millettia root twigs with semi-lignifying stems asexplants; performing tissue culture, thereby acquiring thick and strong high-quality beautiful millettia root tissue culture seedlings; performing nutrition cultivation for boosting growth and rooting, and then transplanting in a field. According to the invention, raw materials for tissue culture are low in cost, nontoxic and high-quality seedlings can be cultivated at a short time, and the cultivation is not influenced by natural environment, such as weather; the explants are disinfected with hydrogen peroxide and mercury bichloride solution, the browning rate of tissue is low, disinfectionis thorough and pollution in subsequent cultivation process is less; a culture medium used in the tissue culture process is simple in components and is low in cost; seedlings have a high adaptive capacity to environment; survival rate of transplanting every time is high; the used waste culture medium can be reused; use ratio of resources is increased and environment is protected.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation and planting, and in particular relates to a method for cultivating traditional Chinese medicine Niu Dali. Background technique [0002] Niu Dali ( Millettia specisoa Champ. ), also known as pig's foot hat, golden bell root, mountain lotus root, upside down golden bell, and vigorous potato; it is a plant of the genus Fabaceae Tachibana-beautiful Tachibana. Niu Dali is a wild and precious medicinal material distributed in tropical and subtropical regions. Its roots are used as medicine, and its main components are protein, starch and alkaloids. Sweet in taste and flat in nature, it belongs to the lung and kidney channels; it has the effects of nourishing deficiency and moistening lung, strengthening tendons and activating collaterals. For lumbar muscle strain, rheumatoid arthritis, lung heat, lung deficiency cough, tuberculosis, chronic bronchitis, chronic hepatitis, spermatorrhea, leuc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 朱丹王德宝郭宏伟苏志恒康亮
Owner GUANGXI MEDICAL UNIVERSITY
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