Cultivation method for beautiful millettia roots
A breeding method, Niu Dali's technology, which is applied in the field of traditional Chinese medicine Niu Dali's cultivation, can solve the problems of difficult disposal of waste medium, high sugar content in the medium, and complex fungicide components, etc., and achieve easy plant absorption and utilization, and transplant survival The effect of high yield and shortened incubation time
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[0024] A method for cultivating cattle vigorously comprises the following steps:
[0025] (1) Disinfection of explants: Select the twigs of the semi-lignified stems of Niu Dali, rinse them with clean water first, then cut them into small stems with a length of 0.5-1.0 cm, dry the surface water with filter paper, and dry them in a 3% filter paper. Sterilize in hydrogen peroxide solution for 60 seconds, wash twice with sterile water, blot the surface moisture with sterile paper, immerse in 0.1% mercuric acid solution for sterilization for 12 minutes, wash with sterile water for 5 times, use sterile paper Blot surface moisture.
[0026] (2) Induction cultivation: transfer the sterilized explants into the induction medium to induce differentiation, the cultivation conditions: the temperature is 23-27°C, the light time is 12 h / day, and the light intensity is 1600-2000lx; the induction medium is : MS + sucrose 20g / L + carrageenan 4g / L + 6-BA 2mg / L +KT 1mg / L +NAA 0.4mg / L +AC 1g / L, t...
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