A method for testing the developmental toxicity of environmental estrogen to silkworm

An environmental estrogen and testing method technology, which is applied in the field of environmental estrogen to silkworm developmental toxicity testing, can solve the problems of long time consumption, difficult silkworm feeding and operation, etc., and achieves the effect of a sensitive detection method

Active Publication Date: 2022-06-24
SUZHOU UNIV OF SCI & TECH
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

This method of adding 4-NP to the whole age is time-consuming and has certain difficulties in the breeding operation of silkworms.

Method used

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  • A method for testing the developmental toxicity of environmental estrogen to silkworm
  • A method for testing the developmental toxicity of environmental estrogen to silkworm

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Experimental program
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Embodiment Construction

[0022] The present invention will be described in detail below with reference to the accompanying drawings and in conjunction with the embodiments.

[0023] A method for testing the developmental toxicity of environmental estrogen to silkworm, comprising the following steps:

[0024] Step 1) First, 1176 mg of 4-NP was weighed and dissolved in 50 ml of absolute ethanol to prepare a 20 g / L 4-NP solution. The NP mother solution was transferred into the reagent bottle and sealed with a film, and then stored in a 4°C refrigerator away from light;

[0025] Step 2) During the experiment, take out the above-mentioned mother liquor, and after returning to room temperature and shaking well, prepare 100 ml of 4-NP exposure solutions with concentrations of 0.02, 0.20, and 2.0 g / L in ultrapure water and absolute ethanol, so that each The concentration of absolute ethanol in all groups was 10%, and the reagent bottles were sealed with film and stored in a 4°C refrigerator away from light; ...

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Abstract

The invention discloses a method for testing the developmental toxicity of environmental estrogens to silkworms, which comprises the following steps: firstly, the 5th instar silkworm larvae containing a series of concentrations of test substances are starved for 2 hours by oral gavage injection; Stomach of the silkworm was dissected to obtain tissues and organs. Toxicity endpoints include glutathione reductase activity coefficient and anti-superoxide anion free radical activity in silkworm midgut. The toxicity of the test substance to silkworm development was determined by comparing the toxicity endpoints of the exposure group and the control group. To study the effects of environmental estrogen 4‑nonylphenol on midgut development in silkworm larvae to determine its dose-effect relationship on developmental toxicity in silkworm larvae. By adopting the technical scheme of the invention, multiple biological effect endpoints can be produced on the environmental estrogen nonylphenol, and the potential risk of silkworm exposed to the estrogen environmental concentration level can be evaluated, which is a relatively fast, simple and sensitive detection method.

Description

technical field [0001] The invention relates to the biological field, in particular to a method for testing the developmental toxicity of environmental estrogens to silkworms. Background technique [0002] Environmental hormones are synthetic chemicals that are widely present in nature and exhibit estrogenic effects on invertebrates and vertebrates. Nonylphenol (NP) is a substance that has been shown to have estrogenic effects. NP has been widely used in industrial and agricultural production processes, is stable at room temperature, and is not easily degraded. In recent years, NPs have been detected in many rivers and lakes such as Taihu Lake and Songhua River Basin in my country. NPs were also detected in organisms such as tap water, milk, animals and plants. NPs can enter into organisms through various ways and have bioaccumulation. The permanent nature and lipophilicity of NPs lead to their easy accumulation in animal tissues, where both acute and chronic toxic effec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/74
CPCG01N33/74
Inventor 袁红霞秦粉菊
Owner SUZHOU UNIV OF SCI & TECH
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