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Agar gel immunodiffusion test punching device

An immunodiffusion and hole punch technology, which is applied in the field of immunological testing technology research and teaching, can solve the problems of easy aging of rubber airbags, increase workload, affect test results, etc., and achieve suitable promotion and use, reduce workload, and facilitate operation Effect

Pending Publication Date: 2019-04-05
HEFEI CAREER TECHNICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this punch is that several holes need to be punched for the agar immunodiffusion test. With this punch, only one hole can be punched, which is time-consuming and laborious, and it is easy to miss the hole or not make a complete hole, which affects the test. result
The rubber airbag of the existing puncher is easy to age. After the hole is punched, the agar blocks in the holes on the agar plate cannot be sucked out in time, and even need to be picked out one by one with a needle; the residual agar blocks in the puncher are not easy to remove, which increases the workload

Method used

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  • Agar gel immunodiffusion test punching device
  • Agar gel immunodiffusion test punching device
  • Agar gel immunodiffusion test punching device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] This embodiment is used as a single-hole puncher. In specific use, the one-way agar diffusion test is used to detect the antigen concentration. A single-hole puncher can be used to connect the internal thread 31 on the inner wall of the adapter head 3 to the bottom end of the outer wall of the sealed air chamber 2. The external thread 26 is connected, and the perforated tube 4 is directly inserted in the center hole 32 of the conversion head 3, and the other six perforated tube clamping grooves 34 are sealed with sealing rings. Before drilling, press the rebound device 1 for the first time, press the rebound device 1 and push the piston 23 to the lower end of the sealed air chamber 2, and discharge part of the air in the sealed air chamber 2; Press the rebound device 1 down, press the rebound device 1 to bounce back, and pull the piston 23 to the upper part of the sealed air chamber 2 through the spring 24, and the air pressure formed in the sealed air chamber 2 makes th...

Embodiment 2

[0037] Two-way agar diffusion test was used to analyze the nature of the antigen. Three holes were drilled on an agar plate in a triangular arrangement, two of which were added with the antigen to be tested and the standard antigen, and the remaining one with the antibody. Two precipitation lines coincide with each other, indicating that the two antigens are completely identical in nature; if two precipitation lines are partially connected, it indicates that the two antigens are partially identical; if two precipitation lines cross, it indicates that the two antigens are completely different.

[0038] This embodiment is used as a three-hole puncher. In specific use, connect the internal thread 31 on the inner wall of the conversion head 3 with the external thread 26 on the bottom end of the outer wall of the sealed air chamber 2, and insert the three pipe holes 4 into three equilateral triangle holes. In the punching hole slot 34, the hole distance needs to be adjusted accordin...

Embodiment 3

[0041] Use the two-way agar diffusion test to detect the antibody titer. Drill 7 holes in the shape of a quincunx on the gel plate, add quantitative antigen to the central hole, and add antibodies of different dilutions to the peripheral holes. The antibody dilution with the highest precipitation line is the Antibody titer.

[0042] This embodiment is used as a three-hole puncher. In specific use, the internal thread 31 on the inner wall of the adapter 3 is connected to the external thread 26 on the bottom end of the outer wall of the sealed air cavity 2, and the six pipe holes 4 are inserted into the six pipe holes. In the slot 34, another perforated tube 4 is inserted into the center hole 32, and the hole distance needs to be adjusted according to the experimental requirements. The spacing adjustment range of the perforated tube is between 3mm-5mm. The line 44 of the first scale on the conversion head 3 is aligned with the second scale 35 on the conversion head 3. According ...

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Abstract

The invention relates to an agar gel immunodiffusion test punching device. The agar gel immunodiffusion test punching device comprises a sealing gas cavity, a switching head and a punching pipe; the sealing gas cavity is of a barrel-shaped structure of which the upper and lower ends are open; a pressing and bouncing device is arranged on the middle upper part of the sealing gas cavity; the bottomend of the pressing and bouncing device is fixedly connected to a piston through a spring; the piston is movably connected into the sealing gas cavity; outer threads are formed in the bottom end of the outer wall of the sealing gas cavity; an infrared temperature sensor is arranged on the side of the sealing gas cavity; the switching head is of a barrel-shaped structure of which is upper end is open; inner threads which are matched with the outer threads are formed in the upper end of the inner wall of the switching head; an edge sealing ring is arranged on the outer side edge of the switchinghead; a central hole is formed in the center of the bottom wall of the switching head; the punching pipe is of an integral structure consisting of a front part barrel-shaped pipe and a tail part round sheet; the front part barrel-shaped pipe penetrates through the tail part round sheet; the diameter of the front part barrel-shaped pipe is matched with the inner diameter of the central hole; and asealing ring is further arranged on the edge of the tail part round sheet. The agar gel immunodiffusion test punching device is simple in structure and is suitable for promotion and use.

Description

technical field [0001] The invention relates to the technical field of scientific research and teaching of immunological testing technology, in particular to an agar immunodiffusion test hole puncher. Background technique [0002] The soluble antigen and the corresponding antibody diffuse in the agar gel to form a concentration gradient, and a visible precipitation line or precipitation ring is formed at the position where the antigen and antibody meet and the concentration ratio is appropriate. Agar immunodiffusion test is divided into two-way agar immunodiffusion test and one-way agar immunodiffusion test, which are commonly used in serum detection, agar diffusion, antibiotic sensitivity and other tests. Commonly used gels are agar, agarose, dextran or polyacrylamide gels. The gel diffusion test needs to use a hole puncher to punch holes that meet the test requirements. The test generally needs to punch out double holes, double rows of holes, three-hole squares, and seven...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B26F1/02B26D7/00B26D7/26G01N33/559
CPCB26F1/02B26D7/00B26D7/2628B26D2007/0012G01N33/559
Inventor 苏琰李融杜江杨娜娜代永沛
Owner HEFEI CAREER TECHNICAL COLLEGE
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