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Peripheral blood culture medium and preparation method thereof

A culture medium and peripheral blood technology, applied in the medical field, can solve the problems of high requirements, cumbersome preparation process, and complex composition of peripheral blood culture medium, and achieve the effects of low cost, simple and scientific process, and reasonable process design

Inactive Publication Date: 2019-04-05
苏州荣世医药科技有限公司
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

[0002] The composition of common peripheral blood culture medium is complex, the preparation process is cumbersome, and the preparation process has high requirements on the environment, which is not conducive to the large-scale preparation of peripheral blood culture medium

Method used

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specific Embodiment

[0021] In a specific embodiment, a peripheral blood culture medium is characterized in that: it is prepared from the following components:

[0022] RPMI 1640 12-15 grams;

[0023] 600-800ml deionized water;

[0024] Penicillin 100 IU / ML;

[0025] Streptomycin 100IU / ML;

[0026] Sodium bicarbonate solution concentration is 5%;

[0027] The concentration of colchicine in 50ml is 0.04mg per ml;

[0028] 250ml bovine serum;

[0029] Phytohemagglutinin 18-22 sticks.

[0030] The preparation method of a kind of peripheral blood culture medium is characterized in that its steps are:

[0031] (1) Dissolve 12-15 grams of RPMI 1640 in 600-800 ml of deionized water;

[0032] (2), then add 100 IU / ML penicillin and 100 IU / ML penicillin streptomycin to the deionized water prepared in step (1);

[0033] (3), then use a concentration of 5% sodium bicarbonate solution to adjust the pH value of the solution prepared in step (2) to between 7.5-7.8;

[0034] (4), then add 50 milliliters ...

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Abstract

The invention relates to the technical field of medical treatment, and in particular relates to a peripheral blood culture medium and a preparation method thereof. The peripheral blood culture mediumis prepared by reasonable process steps with common RPMI 1640, deionized water, penicillin, streptomycin, a sodium hydrogen carbonate solution, colchicine, bovine serum and phytohemagglutinin as majorraw materials. In addition, the peripheral blood culture medium and the preparation method thereof have the advantages of reasonable process design, simple and scientific process, and relatively lowcost of preparation process, and are suitable for popularization and use.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a peripheral blood culture medium and a preparation method thereof. Background technique [0002] The composition of common peripheral blood culture medium is complex, the preparation process is cumbersome, and the preparation process has high requirements on the environment, which is not conducive to the large-scale preparation of peripheral blood culture medium. [0003] In order to solve the above-mentioned technical problems, the present invention designs a kind of peripheral blood culture medium and its preparation method, and this peripheral blood culture medium and its preparation method use common RPMI 1640, deionized water, penicillin, streptomycin, sodium bicarbonate solution , colchicine, bovine serum and phytohemagglutinin are the main raw materials, and the peripheral blood culture medium is prepared through reasonable process steps. In addition, the peripheral blo...

Claims

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Application Information

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IPC IPC(8): C12N5/078
CPCC12N5/0634C12N2500/60C12N2501/998C12N2501/999
Inventor 朱华东
Owner 苏州荣世医药科技有限公司
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