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Preparation method of asparagus agar oligosaccharide

A technology of agar oligosaccharides and asparagus, which is applied in the field of oligosaccharide production, can solve the problems of low extraction rate of agar, low activity of beneficial bacteria, environmental pollution, etc., and achieve improved extraction rate of agar, high activity of beneficial bacteria, The effect of high antioxidant activity

Inactive Publication Date: 2019-04-05
福建大昌生物科技实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In the prior art, the extraction of agar oligosaccharides usually uses a chemical method or an enzymatic hydrolysis method to extract the agar. The chemical method uses strong acid and strong alkali chemical substances when extracting the agar, such as the patent application number CN201510019814.4 discloses a kind of Gracilaria agarose oligosaccharide and its preparation method, the safety of the production process is poor, and it will cause environmental pollution
Both the chemical method and the enzymatic hydrolysis method have the defects of low agar extraction rate, poor antioxidant activity and low probiotic activity of the produced agar oligosaccharides

Method used

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  • Preparation method of asparagus agar oligosaccharide
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  • Preparation method of asparagus agar oligosaccharide

Examples

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Embodiment 1

[0026] The present invention is a kind of preparation method of asparagus agar oligosaccharide, comprising the following steps:

[0027] (1) After soaking asparagus in salt water for 3 hours, wash, dry and sterilize;

[0028] (2) Crushing the asparagus tubers in a grinder, extruding and filtering through a filter screen with a mesh number of 100 mesh to obtain asparagus vegetable puree;

[0029] (3) Add asparagus vegetable puree to water, the mass ratio of asparagus vegetable puree to water is 1:21, mix well and then heat, and heat at 105°C for 90 minutes to obtain a water extraction mixture;

[0030] (4) Microwave heating the water-extraction mixture, the microwave heating power is high, and the microwave heating is intermittent heating, heating for 1 minute, stopping for 4 minutes, repeating 3 times, and then extruding the gel to obtain an agar solution;

[0031] (5) Cool the agar solution to room temperature and add a buffer to make the pH of the agar solution 6, then add ...

Embodiment 2

[0034] The present invention is a kind of preparation method of asparagus agar oligosaccharide, comprising the following steps:

[0035] (1) After soaking asparagus in salt water for 5 hours, wash, dry and sterilize;

[0036] (2) Break the asparagus tubers in a grinder, squeeze and filter through a filter screen with a mesh number of 150 mesh, and obtain asparagus vegetable puree:

[0037] (3) Add asparagus vegetable puree to water, the mass ratio of asparagus vegetable puree to water is 1:26, mix well and then heat, and heat at 115°C for 105min to obtain the water extraction mixture;

[0038] (4) Microwave heating the water-extraction mixture, the microwave power is medium heat, and the microwave heating is intermittent heating, heating for 2 minutes, stopping for 6 minutes, repeating 5 times, and then extruding the gel to obtain an agar solution;

[0039] (5) Cool the agar solution to room temperature and add a buffer to make the pH of the agar solution 8, then add β-agaras...

Embodiment 3

[0042] The present invention is a kind of preparation method of asparagus agar oligosaccharide, comprising the following steps:

[0043] (1) After soaking asparagus in salt water for 4 hours, wash, dry and sterilize;

[0044] (2) Break the asparagus tubers in a grinder, squeeze and filter through a filter screen with a mesh number of 120 mesh, and obtain asparagus vegetable puree:

[0045] (3) Add asparagus vegetable puree to water, the mass ratio of asparagus vegetable puree to water is 1:24, mix well and then heat, and heat at 110°C for 100 minutes to obtain a water extraction mixture;

[0046] (4) Microwave heating the water-extraction mixture, the microwave power is medium heat, high heat, microwave heating is intermittent heating, heating for 1.5min, stopping for 5min, repeating 4 times, and then extruding the gel to obtain the agar solution ;

[0047] (5) Cool the agar solution to room temperature and add a buffer to make the pH of the agar solution 7, then add β-agara...

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Abstract

The invention provides a preparation method of asparagus agar oligosaccharide, and relates to the technical field of oligosaccharide production. The preparation method comprises the following steps: (1) soaking the asparagus in salt brine for 3 to 5 hours, and then drying the asparagus; (2) pulverizing the asparagus in a pulverizer and filtering the smashed asparagus to obtain asparagus puree; (3)adding the asparagus puree into water at the mass ratio of the asparagus puree to the water tobe 1 to (21-26), and heating the mixture at the temperature of 105-115 DEG C for 90-105 min to obtain a water-extracted mixture; (4) carrying out microwave heating and agar extraction on the water-extracted mixture to obtain an agar solution; (5) after cooling, adding a buffer agent and beta-agarase, heating the mixture to 38-45 DEG C, and carrying out enzymolysis on the mixture for 3-10 h to obtain an asparagus oligosaccharide solution; and (6) drying the asparagus oligosaccharide solution to obtainthe asparagus oligosaccharide. According to the invention, the agar is extracted by the process of crushing, heating and microwave treatment of the asparagus, the extraction rate of the agar is increased and the prepared agar oligosaccharide has high antioxidant activity and high probiotics activity.

Description

technical field [0001] The invention relates to the technical field of oligosaccharide production, in particular to a preparation method of asparagus agar oligosaccharide. Background technique [0002] Asparagus is a kind of seaweed plant. The algae are reddish brown. Most of them grow on disc-shaped holders, growing linearly and upright in a cylindrical shape. The mature algae have long branches, and the upper part of the branches often grows into a whip. The algae like to grow in the sand swamp in the lower part of the intertidal zone and above and below the rocks covered with sand. When the temperature is not suitable, the exposed part will die. When the temperature is suitable for its growth, asparagus will grow again. New algae branches can be planted in large areas in my country at present. [0003] Asparagus contains polysaccharides, phycobiliproteins and amino acids, and the former two account for the vast majority. [0004] Among them, sugars accounted for the larg...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/00C08B37/12
CPCC12P19/00C12P19/14C08B37/0039
Inventor 吴云通黄贞胜欧婧
Owner 福建大昌生物科技实业有限公司
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