Dry recovery device of cell cryopreservation bags and working method

Abstract

The invention discloses a dry recovery device of cell cryopreservation bags, comprising a working cabin body, an air exhaust device, temperature measuring devices, a built-in rocker shaker, an elasticcover net, a temperature control device, a hot air flow generating device, ozone generating devices, a numerical control device and an operation interface; the working cabin body comprises a workingcabin main body and a cabin cover movably connected to the working bin main body; the air exhaust device is fixedly mounted on the lateral side of the working cabin body, the temperature measuring devices are arranged inside and outside the working cabin body, and the built-in rocker shaker is installed inside the working cabin body; the built-in rocker shaker is flexibly connected to the elasticcover net, and the temperature control device is installed in the built-in rocker shaker; the upper part of the built-in rocker shaker is equipped with the hot air flow generating device, and the hotair flow generating device is disposed in the cabin cover; and the ozone generating devices are disposed in the working cabin main body and the cabin cover, the numerical control device is installed below the built-in rocker shaker, and the operation interface is arranged outside the working cabin body.

Description

technical field [0001] The invention relates to the field of cell resuscitation, in particular to a cell cryopreservation bag dry resuscitation device and a working method. Background technique [0002] With the development of life science and technology, cell therapy technology is changing with each passing day, mainly including stem cell therapy and immune cell therapy represented by CAR-T cells. The process of cell product preparation involves freezing and thawing of cells. When the cells are thawed, the best operation is to quickly raise the temperature of the cell cryopreservation bag just taken out of the liquid nitrogen tank to 37°C, so that it can quickly pass through the most vulnerable -5°C to 0°C to avoid ice crystal recrystallization. The cell causes damage, leading to the death of the cell. [0003] There are two common types of cryopreservation containers: cell cryopreservation tubes and cell cryopreservation bags. The cryopreservation capacity of cell cryop...

AI Technical Summary

Problems solved by technology

At present, the resuscitation of cell cryopreservation bags can be carried out in a water bath resuscitation method or a dry resuscitation method. The former water bath resuscitation method requires a full manual operation, cannot implement standardized operations, and is easy to contaminate cells. It has been gradually eliminated, while the latter dry resuscitation method At present, the products are limited, the recovery method is single, the recovery efficiency is not satisfactory, and there is still a lot of improvement and development needs

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