A kind of preparation method of mannan with specific molecular weight

A mannan, molecular weight technology, applied in biochemical equipment and methods, biological material sampling methods, bioreactors/fermenters for specific purposes, etc., can solve problems such as limited application, low efficiency, and residual degradation products, To achieve the effect of simplifying the production process, improving the enzymatic hydrolysis rate and shortening the time

Active Publication Date: 2022-06-21
赵勤农
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

By reducing the substrate concentration, agglomeration can be avoided, but it is very inefficient when used in large-scale production; stirring is inefficient in large-scale production, and agglomeration still occurs due to uneven stirring; adding ions or using Some reagents are processed and easily remain in the degradation products, which increases the difficulty of purification and limits its application in the fields of food and medicine

Method used

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  • A kind of preparation method of mannan with specific molecular weight

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Comparison scheme
Effect test

Embodiment 1

[0026] Inject 450kg of production water into the enzymolysis vessel, adjust the temperature in the enzymolysis vessel to 30°C, pH to 6.0-7.0, and put neutral β-mannanase into the enzymolysis vessel (the amount of enzyme input is based on the quality of the enzyme material). The ratio is 1:15), then weigh 50kg of konjac powder and put it into the enzymolysis container. Under the impetus of the twin-screw propulsion device and the gear pump, the enzymolysis cycle is carried out, and the vibration probe of the digital display device for molecular weight online detection is adjusted. The frequency is 80Hz and the amplitude is 1mm. As the enzymolysis proceeds, the viscosity of the enzymolysis solution gradually decreases. When the molecular weight online detection digital display device detects that the molecular weight of the enzymolysis solution is 100000-200000Da, the digital display temperature control system is set according to the setting. The program automatically activates t...

Embodiment 2

[0028] Inject 450kg of production water into the enzymolysis vessel, adjust the temperature in the enzymolysis vessel to 30°C, pH to 6.0-7.0, and put neutral β-mannanase into the enzymolysis vessel (the amount of enzyme input is based on the quality of the enzyme material). The ratio is 1:15), then weigh 50kg of guar gum, put it into the enzymolysis container, carry out cyclic enzymolysis under the impetus of twin-screw propulsion device and gear pump, and adjust the vibration of the digital display device for molecular weight online detection. The probe frequency is 80Hz and the amplitude is 1mm. As the enzymolysis progresses, the viscosity of the enzymolysis solution gradually decreases. When the molecular weight online detection digital display device detects that the molecular weight of the enzymolysis solution is 10000-12000Da, the digital display temperature control system will be set according to the setting. The predetermined program automatically activates the heating ...

Embodiment 3

[0030]Inject 450kg of production water into the enzymolysis vessel, adjust the temperature inside the enzymolysis vessel to 30°C, pH to 6.0-7.0, and put neutral β-mannanase into the enzymolysis vessel (the amount of enzyme input is based on the quality of the enzyme material). The ratio is 1:15), then weigh 50kg of locust bean gum and put it into the enzymolysis vessel. Under the impetus of the twin-screw propulsion device and the gear pump, carry out cyclic enzymolysis and adjust the vibration probe of the digital display device for molecular weight online detection. The frequency is 50Hz, and the amplitude is 2mm. As the enzymolysis proceeds, the viscosity of the enzymolysis solution gradually decreases. When the molecular weight online detection digital display device detects that the molecular weight of the enzymolysis solution is 3000-5000Da, the digital display temperature control system is set according to the setting. The program automatically activates the heating func...

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Abstract

The invention discloses a method for enzymolyzing hemicellulose to prepare mannan with a specific molecular weight. A special enzymolysis system is mainly used for controllable enzymolysis. The enzymolysis system includes an enzymolysis container and a twin-screw propulsion device , Digital display temperature control system, molecular weight online detection digital display device, gear pump. Using this enzymatic hydrolysis system can accurately control the degree of enzymatic hydrolysis to obtain mannan with the required molecular weight or molecular weight range; this enzymatic hydrolysis system solves the problems of high viscosity during the enzymatic hydrolysis of hemicellulose and easy caking when feeding , the rate of enzymatic hydrolysis is improved. Under the same conditions, compared with the unimproved enzymatic hydrolysis device, the enzymatic hydrolysis process shortens the time by one-third to one-half; at the same time, enzymatic hydrolysis and deactivation can be carried out in the same container , no need for external enzyme inactivation equipment, which simplifies the production process and increases efficiency and energy saving.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and in particular relates to a preparation device and method of mannan with a specific molecular weight. Background technique [0002] Glycans are linear polysaccharides linked by β-1,4-D-mannopyranosidic bonds, if certain residues in the main chain are replaced by glucose, or galactose is linked to mannose by 1,6-α-glycosidic bonds The residues are connected to form branches, which are called isomannans, mainly galactomannans, glucomannans and galactoglucomannans. Due to the different types of monosaccharides, degree of polymerization, sugar bond connection and arrangement of these glycans, different glycans have obvious differences in solubility, viscosity and other properties, which greatly affects the β-mannan Carbohydrase hydrolysis. Therefore, the application of mannan is limited to a certain extent. [0003] In the research of mannanase-catalyzed degradation, most of the researches f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P19/04C12M1/40C12M1/38C12M1/34
CPCC12M21/18C12M33/16C12M41/12C12M41/30C12P19/04C12P19/14
Inventor 钱金宏卢晓会钱乾向立军张冉钱杰瑞
Owner 赵勤农
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