Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of preparation method and application of high-purity mannoglycan

A mannanase, high-purity technology, applied in the field of preparation of high-purity mannan, can solve the problems of increased solubility, long irradiation time, and reduced viscosity of KGM hydrosol

Active Publication Date: 2020-07-07
NINGBO BAIERMA BIOLOGICAL TECH CO LTD
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Chinese patent (Application No. 201310022943.X) discloses a method for synergistically preparing konjac glucomannan with a medium degree of polymerization, and mentions a medium degree of polymerization KGM whose degree of polymerization is between 100 and 900. Under the premise of maintaining the original characteristics of KGM, the viscosity of KGM hydrosol is reduced, and the solubility of KGM is effectively increased. However, this method uses irradiation to degrade glucomannan, and the irradiation time is long, and each test The processing capacity is small, which is not conducive to large-scale industrial production
[0006] Chinese patent (application number 201110309468.5) discloses a preparation method of konjac glucomannan and glucomannan oligosaccharides with different molecular weights, and proposes to degrade glucomannan into enzymatic hydrolysates of different molecular weights, but does not specify the enzyme The degree of polymerization of the hydrolyzate does not define the functional characteristics of the enzymatic hydrolyzate of different molecular segments, and the process is simple, only after preliminary alcohol precipitation separation, the purity of the product is low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of preparation method and application of high-purity mannoglycan
  • A kind of preparation method and application of high-purity mannoglycan
  • A kind of preparation method and application of high-purity mannoglycan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: as figure 1 Shown is a preparation method of mannan, comprising the following steps:

[0035] (1) Add 200kg of production water to the enzymolysis tank, control the temperature in the enzymolysis tank to 30-50°C, and put neutral β-mannanase into the enzymolysis tank (the amount of enzyme input is based on the amount of enzyme and konjac extract Powder mass ratio is 1:10-30), weigh 10kg of konjac fine powder, put it evenly into the enzymolysis tank with rapid and automatic detection material viscosity device, control the pH of the material in the enzymolysis tank to 6.0-7.5, with the enzyme As the hydrolysis time advances, the viscosity of the enzymolysis solution drops rapidly. When the viscosity real-time detection device installed on the enzymolysis tank detects that the viscosity of the enzymolysis solution reaches 1100-1200mpa.s, the enzyme is immediately deactivated, and the deactivation time is 20-30min. cool down;

[0036] (2) Microfiltration to remo...

effect experiment Embodiment example 1

[0062] Effect Experiment Implementation Case 1: Mannoside Glycan Used in Anti-tumor Experiment

[0063] An application of the above-mentioned produced mannosidose in anti-tumor. Mannosidose is made into corresponding products or applied in anti-tumor products, which has obvious inhibitory effect on tumors. The ratio to body weight is 100-300mg / kg.

[0064] Anti-tumor test plan: select mice inoculated with tumors for 10 days, aseptically extract ascites from mice, dilute with 0.9% sodium chloride injection 3 times, adjust the number of tumor cells to 1×108 / ml, and give each mouse axillary Inoculate 0.2ml subcutaneously. Randomly grouped into groups, and started administration 24 hours after inoculation. The control group received the same amount of normal saline, once a day; the 5-fluorouracil group was 10 mg / kg, subcutaneously injected, once a week; the product of Example 1 and the product of Comparative Example 1 1. Each 200 mg / kg product of Comparative Example 2 was admini...

effect experiment Embodiment example 2

[0069] Effect experiment implementation case 2: Antiviral test of mannosidin sulfated derivatives

[0070] An application of the above-mentioned produced mannosidose in anti-virus, wherein the mannosidose is subjected to sulfate modification treatment to make corresponding products or apply it to anti-virus products, so that the sulfated mannosidose When the concentration is above 200μg / ml, it has a good broad-spectrum antiviral effect.

[0071] Sulfated polysaccharides refer to natural and semi-synthetic acidic polysaccharides containing sulfuric acid groups, which are polyanionic compounds. Since the discovery of sulfated dextran in 1987 that it has the activity of inhibiting AIDS virus HIV, the antiviral aspects of this type of polysaccharides The research is very active, and it may become another class of HIV-1 inhibitors following viral reverse transcriptase activity inhibitors and protease inhibitors. Sulfated mannan is modified by sulfation of mannan by chlorosulfonic ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
viscosityaaaaaaaaaa
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses high-purity hypo-mannan. Controllable enzymatic hydrolysis is carried out on konjac fine powder by the aid of beta-mannase to generate the high-purity hypo-mannan, and the high-purity hypo-mannan is purified to obtain polysaccharide systems with the weight-average molecular weight of 60000-65000 Da. The high-purity hypo-mannan has the advantages that corresponding productscan be prepared from the polysaccharide systems, or the polysaccharide systems can be applied to antitumor products to be administered, and accordingly obvious effects of suppressing tumor can be realized; sulphating and derivatization treatment can be carried out on the high-purity hypo-mannan to obtain corresponding products, or the high-purity hypo-mannan can be applied to antiviral products, and accordingly excellent broad-spectrum antiviral effects can be realized.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and in particular relates to a preparation method of high-purity mannan and its application in the field of medicine. Background technique [0002] In recent years, research on functional polysaccharides has been very active at home and abroad. Many developed countries have designated a variety of polysaccharide products as specific health foods to prevent and treat obesity, hyperglycemia, hyperlipidemia, arteriosclerosis and coronary heart disease. [0003] Konjac glucomannan (KGM) is a polysaccharide formed by combining D-glucose and D-mannose through β-1, 4 glycosidic bonds, and its average degree of polymerization is between 1000 and 10000. Because KGM has defects such as high degree of molecular polymerization, high viscosity, and low solubility, the application of natural KGM is limited to a certain extent. [0004] KGM can be degraded to produce glucomannan with different degrees of po...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C08B37/00A61P35/00
CPCC08B37/0003C12P19/04
Inventor 应烨钱金宏卢晓会钱乾
Owner NINGBO BAIERMA BIOLOGICAL TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com