Preparation method of high-purity mannan and application of high-purity mannan

A mannanase and high-purity technology, which is applied in the field of preparation of high-purity mannan, can solve the problems of small test treatment volume, long irradiation time, and low purity, and achieve important economic and production values ​​and operational processes. Simple, optimized preparation process effect

Active Publication Date: 2018-04-20
NINGBO BAIERMA BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Chinese patent (Application No. 201310022943.X) discloses a method for synergistically preparing konjac glucomannan with a medium degree of polymerization, and mentions a medium degree of polymerization KGM whose degree of polymerization is between 100 and 900. Under the premise of maintaining the original characteristics of KGM, the viscosity of KGM hydrosol is reduced, and the solubility of KGM is effectively increased. However, this method uses irradiation to degrade glucomannan, and the irradiation time is long, and each test The processing capacity is small, which is not conducive to large-scale industrial production
[0006] Chinese patent (application number 201110309468.5) discloses a preparation method of konjac glucomannan and glucomannan oligosaccharides with different molecular weights, and proposes to degrade glucomannan into enzymatic hydrolysates of different molecular weights, but does not specify the enzyme The degree of polymerization of the hydrolyzate does not define the functional characteristics of the enzymatic hydrolyzate of different molecular segments, and the process is simple, only after preliminary alcohol precipitation separation, the purity of the product is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: as figure 1 Shown is a preparation method of mannan, comprising the following steps:

[0036] (1) Add 200kg of production water to the enzymolysis tank, control the temperature inside the enzymolysis tank to be 30-50°C, and put neutral β-mannanase into the enzymolysis tank (the amount of enzyme input is based on the amount of enzyme and konjac extract Powder mass ratio is 1:10-30), weigh 10kg of konjac fine powder, put it evenly into the enzymolysis tank with rapid and automatic detection material viscosity device, control the pH of the material in the enzymolysis tank to 6.0-7.5, with the enzyme As the hydrolysis time advances, the viscosity of the enzymolysis solution drops rapidly. When the viscosity real-time detection device installed on the enzymolysis tank detects that the viscosity of the enzymolysis solution reaches 700-800mpa.s, the enzyme is immediately deactivated, and the deactivation time is 20-30min. cool down;

[0037] (2) Microfiltration to...

effect experiment Embodiment example 1

[0063] Effect experiment implementation case 1: Mannosidan used in hypoglycemic experiment

[0064] Experimental method: Select 60 Kunming adult mice (half male and half male), with an average body weight of (25±2.5) g, randomly select 10 mice as the normal control group according to the principle of half male and half male, and feed the remaining 50 mice with high-sugar feed for 1 -After 2 months, diabetes was induced with small doses of streptozotocin, and those whose fasting blood glucose value was greater than 20mmol / L were included in the experiment, and then the mice were randomly divided into a type II diabetes model group, a metformin administration group, and the product of Example 1 The administration group, the product administration group of Comparative Example 1 and the product administration group of Comparative Example 2; the normal control group and the type II diabetes model group were given the same amount of distilled water. The experimental period was 4 wee...

effect experiment Embodiment example 2

[0071] Effect Experiment Implementation Case 2: Anticoagulant Test of Mannan Sulfated Derivatives

[0072] experimental method:

[0073] The products of Example 1, Comparative Example 1, and Comparative Example 2 were subjected to sulfate modification treatment to obtain mannan sulfated derivatives with good water solubility.

[0074] Select 60 Kunming adult mice (half male and half male), with an average body weight of (25±2.5) g, randomly divide them into 5 groups, 12 mice in each group. Gavage according to the capacity of 25ml / kg, respectively give normal saline, warfarin, the sulfated derivative of the product of embodiment 1, the sulfated derivative of the product of comparative example 1, and the sulfated derivative of the product of comparative example 2, each 10mg / kg , continuous gavage for 30d. One hour after administration on the 30th day, blood was collected from the venous plexus behind the inner canthus of the mouse eyes, and the coagulation time of the mice in ...

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Abstract

The invention discloses high-purity mannan. The high-purity mannan is produced by beta-mannase controllable enzymolysis of konjak refined powder and is purified to obtain a polysaccharide system of which the weight average molecular weight is 20000-25000Da. After the polysaccharide system is applied to a product for reducing blood glucose, the blood glucose can be obviously reduced by eating the product; and after the polysaccharide system is subjected to derivatization treatment, the polysaccharide system has anti-coagulation and anti-thrombus effects and is an important heparitin raw material.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and in particular relates to a preparation method of high-purity mannan and its application in the field of medicine. Background technique [0002] In recent years, research on functional polysaccharides has been very active at home and abroad. Many developed countries have designated a variety of polysaccharide products as specific health foods to prevent and treat obesity, hyperglycemia, hyperlipidemia, arteriosclerosis and coronary heart disease. [0003] Konjac glucomannan (KGM) is a polysaccharide formed by combining D-glucose and D-mannose through β-1, 4 glycosidic bonds, and its average degree of polymerization is between 1000 and 10000. Because KGM has defects such as high degree of molecular polymerization, high viscosity, and low solubility, the application of natural KGM is limited to a certain extent. [0004] KGM can be degraded to produce glucomannan with different degrees of po...

Claims

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Application Information

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IPC IPC(8): C08B37/02C12P19/14C12P19/04
CPCC08B37/0003C08B37/009C12P19/04C12P19/14
Inventor 钱乾钱金宏卢晓会应烨
Owner NINGBO BAIERMA BIOLOGICAL TECH CO LTD
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