A kind of cryogenic freezing living body preservation method

Abstract

The invention belongs to the technical field of cryopreservation, and proposes a method for preserving the quality of living organisms by low-temperature freezing, comprising the following steps: A, material pretreatment: removing soil and sundries on the surface of fresh plant rhizomes; B, cooling and dehydration treatment: under ventilated conditions, the The fresh plant rhizomes in step A are subjected to gradient cooling treatment to obtain low-temperature dehydrated plant rhizomes below 0°C, and the slowest cooling rate is adopted at 4°C when the gradient cooling is at 4°C and is less than 1.0°C / d; The temperature of the rhizomes of medium and low temperature dehydrated plants is constant, and air is provided to the rhizomes of low temperature dehydrated plants. At the same time, water mist is sprayed periodically to form an ice layer for heat preservation and water replenishment on the surface to obtain frozen living plant rhizomes; D. Heating and water replenishment treatment: under ventilated conditions, The frozen living plant rhizomes in step C are subjected to gradient temperature rise treatment to obtain the preserved plant rhizomes. The invention solves the problem that the traditional cold storage technology cannot realize the recultivation of plant rhizomes after long time cold storage.

Description

technical field [0001] The invention belongs to the technical field of cryopreservation, and relates to a method for preserving the quality of a living body by cryogenic freezing. Background technique [0002] Traditional refrigeration technology can only achieve short-term preservation and preservation of plant branches, fruits, seeds, rhizomes, leaves, etc. When stored for a long time, plants will wilt or even rot, especially after long-term refrigeration. Live and can be used for re-cultivation. This is because plant cells contain a lot of water, and water has the largest density and smallest volume at 4°C, and the increase and decrease of temperature will increase the volume of water, while the traditional cold storage technology mostly cools plants rapidly under airtight and unventilated conditions. Freezing will cause the free water in the plant cells to lose time, and the frozen volume will increase and puncture the cells, and the plant cell structure will be irrever...

AI Technical Summary

Problems solved by technology

[0002] Traditional refrigeration technology can only achieve short-term preservation and preservation of plant branches, fruits, seeds, rhizomes, leaves, etc. When stored for a long time, plants will wilt or even rot, especially after long-term refrigeration. live and can be used for recultivation

This is because plant cells contain a lot of water, and water has the largest density and smallest volume at 4°C, and the increase and decrease of temperature will increase the volume of water, while the traditional cold storage technology mostly cools plants rapidly under airtight and unventilated conditions. Freezing, which causes the free water in the plant cells to be lost in time, and the freezing volume increases to puncture the cells, and the plant cell structure is irreversibly damaged. The integrity of the cell structure is an important condition for quality preservation and preservation of activity.

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