A high-efficiency phenol-degrading bacteria and its application
A phenol-degrading bacteria, high-efficiency technology, applied in bacteria, biological water/sewage treatment, microorganisms, etc., can solve the problems of complex operation, high cost, secondary pollution, etc., and achieve the effect of improving economic benefits
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Embodiment 1
[0025] Isolation and identification of strains
[0026] Take the polluted soil from the pesticide factory, add it to the inorganic salt medium with phenol (200mg / L) as the only carbon source, and cultivate it in a shaking table at a constant temperature of 150r / min at 30°C; take the acclimatization culture solution every 5 days and inoculate it into fresh In the phenol inorganic salt medium containing 200mg / L, the inoculum size is 10% (V / V); after continuous acclimatization for 30 days, the enriched culture is diluted, spread in 200mg / L phenol solid medium, placed in 30 Cultivate in a constant temperature incubator for 2-3 days to form a single colony. Select the colony for purification and culture to obtain a single colony with the same size and shape. Streak it on a slant solid medium and culture it for 2-3 days to obtain the target colony and store it at about 4°C.
[0027] In some embodiments, the inorganic salt medium may include K 2 HPO 4 、KH 2 PO 4 , NaCl, MgSO 4 ...
Embodiment 2
[0046] The growth curve of embodiment 2 bacterial strains
[0047] Pick the 4 ℃ solid medium to save the bacteria Acinetobacter sp.QC-01 ring to 100mg / L phenol medium and cultivate to 10 8 MPN / ml, inoculate the bacterial suspension into a fresh inorganic salt liquid medium according to the inoculation amount of 5% (V / V), place it in a shaker at 30°C and 150r / min after inoculation and cultivate it. Wherein, the initial pH of the liquid medium is 7.0. The growth curve is drawn with time as the abscissa and absorbance OD600 as the ordinate. During this period, samples were taken every 1 h to determine the OD600 value. Strain growth curve as figure 1 As shown, the strain is in the adjustment period within 0-2h, and enters the logarithmic growth phase from the 2nd hour; at the 12th hour, the OD600 value is 0.8, and when the bacteria continue to grow to the 20th hour, the OD600 value is 1.0.
Embodiment 3
[0048] Example 3 The degradation rate of bacterium bead to phenol under different initial phenol concentration conditions
[0049] Pick the 4 ℃ solid medium to save the bacteria Acinetobacter sp.QC-01 ring to 100mg / L phenol solid medium and cultivate to 10 8 MPN / ml, according to the inoculum size of 5% (V / V), the bacterial suspension was inoculated into the fresh inorganic salt medium of 50mg / L, 100mg / L, 200mg / L, 500mg / L, 1000mg / L respectively, inoculated Afterwards, they were cultured in a shaker at 30°C and 150r / min. Among them, the substrate concentration time of the inorganic salt medium is the abscissa, and the phenol degradation rate is the ordinate, and a curve is drawn to investigate the phenol degradation rate of the bacterial beads under different initial phenol concentration conditions. like figure 2 As shown, the strain can degrade phenol at a rate of 100% within the range of the initial phenol concentration of 0 mg / L-200 mg / L.
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