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A method for obtaining submandibular gland of mouse embryo

A technology of mouse embryos and acquisition methods, which is applied in the field of biomedicine, can solve the problems that the submandibular gland glands cannot be completely attached to the tongue, the submandibular gland tissue is incomplete, and there are many steps, so as to reduce operation steps, reduce operation difficulty, and ensure integrity sexual effect

Active Publication Date: 2022-04-19
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the method of bilateral rotation to separate the mandibular cartilage is mostly used, but this method has many steps and is complicated when operating under the microscope. At the same time, the submandibular gland cannot be completely attached to the tongue, and requires high skills and sufficient proficiency. There is a certain success rate, and the submandibular gland tissue taken out is mostly incomplete

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  • A method for obtaining submandibular gland of mouse embryo

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Embodiment 1

[0026] This embodiment provides a method for obtaining the submandibular gland of mouse embryos. The method has simple steps and is easy to operate. The integrity of the extracted submandibular gland tissue is high. Specific steps are as follows:

[0027] Step 1: After the pregnant mouse is killed, the uterus containing the embryo is completely dissociated.

[0028] Step 2: Open the uterine wall and free the embryo completely.

[0029] Step 3: Obtain the jaw tissue from the embryo under a microscope.

[0030] Step 4: Place the mandibular tissue under a microscope, zoom in on the field of view and fix it. After separating the superficial tissue, pinch off the connection between the submandibular gland, the tongue body and the duct, and obtain the complete embryonic submandibular gland.

Embodiment 2

[0032] This embodiment provides a method for obtaining the submandibular gland of mouse embryos. The method has simple steps and is easy to operate. The integrity of the extracted submandibular gland tissue is high. Specific steps are as follows:

[0033] Step 1: C57BL mice with a gestational age of 12 days were killed by cervical dislocation, and the chest and abdomen of the mice were disinfected by soaking in 75% alcohol. The skin, muscles and peritoneum were cut in a straight line from the pubic symphysis in the midline of the mouse abdomen to expose the abdominal cavity to the sword. Under the protrusion, the abdominal skin and muscles are retracted to expose the uterus, the uterus is lifted with small curved forceps, and the free uterus of the mesentery is cut and separated with scissors. During this process, the uterus and embryos are prevented from being squeezed, and the free uterus containing the embryo Place them in a 100mm Petri dish filled with 25mL storage medium,...

Embodiment 3

[0038] This embodiment provides a method for obtaining the submandibular gland of mouse embryos. The method has simple steps and is easy to operate. The integrity of the extracted submandibular gland tissue is high. Specific steps are as follows:

[0039] Step 1: C3H mice with a gestational age of 13 days were killed by cervical dislocation, and the chest and abdomen of the mice were disinfected by soaking in 75% alcohol. The skin, muscles and peritoneum were cut in a straight line from the pubic symphysis in the midline of the abdomen of the mice to expose the abdominal cavity to the sword. Under the protrusion, the abdominal skin and muscles are retracted to expose the uterus, the uterus is lifted with small curved forceps, and the free uterus of the mesentery is cut and separated with scissors. During this process, the uterus and embryos are prevented from being squeezed, and the free uterus containing the embryo Place them in a 100mm petri dish filled with 25mL storage med...

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Abstract

The invention discloses a method for obtaining the submandibular gland of mouse embryos, and relates to the field of biomedicine. The method includes: freeing the uterus: after killing the pregnant mouse, freeing the uterus containing the embryo completely; freeing the embryo: opening the uterine wall, freeing the embryo completely; obtaining embryonic jaw tissue: obtaining the jaw tissue from the embryo under a dissecting microscope ;Obtain embryonic submandibular gland: place the mandibular tissue under a dissecting microscope, zoom in on the field of view clearly and then fix it. After separating the most superficial tissue, pinch off the connection between the submandibular gland, the tongue body and the duct, and then obtain the complete embryonic submandibular gland. This method improves the operation of obtaining the submandibular gland of mouse embryos. Compared with the traditional method, the operation steps are reduced, the operation difficulty is reduced, and the integrity of the extracted submandibular gland tissue is guaranteed to a greater extent, thus providing a better method. The model of submandibular gland cultured in vitro provides a reliable platform for the research of submandibular gland related diseases.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for obtaining the submandibular gland of mouse embryos. Background technique [0002] The submandibular glands are the salivary glands in the mandible, one on the left and one on the left, and are part of the salivary glands. Their main function is to secrete saliva. The submandibular gland is located under the jawbone (mandible), near the curved angle of the mandible, so it is also called the submandibular gland (the salivary gland under the mandible). As an important part of the salivary glands, the submandibular gland maintains oral health by providing saliva to maintain the pH of the mouth and kill harmful bacteria. Currently, the quality of life of patients with loss of salivary gland function or surgical removal of salivary glands is significantly reduced. Therefore, the study of the branching morphogenesis of the submandibular gland in the mouse embryonic process and ...

Claims

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Application Information

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IPC IPC(8): C12N5/073C12N5/071
CPCC12N5/0603C12N5/0633C12N2509/10
Inventor 刘志凯李春洁吴樱仪曹钰彬
Owner SICHUAN UNIV
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