The invention discloses a conditioning wine, and a preparation method and a drinking method thereof. Through repeated drinking tests, the conditioning wine can enhance human body immunity, conditions endocrine, and can be taken by both men and women; when taken by the women, the wine can nourish ovary, promote female vaginal muscle contraction, warm uterus, promote the uterine wall to be thickened and protect the reproductive system; when taken by the men, the wine can invigorate kidney and strengthen yang, regulate physiological functions, enhance the vitality of sperms and increase the number of the sperms; and the wine also has cancer-prevention, anti-cancer, face-beautifying and anti-aging efficacies, and has special curative effects on hyperlipemia, hyperglycemia, deficiency of both qi and blood, infertility due to uterine coldness, rheumatalgia, men and women poor physique, insomnia and the like.