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Method for culturing photosynthetic microalgae

A cultivation method and photosynthetic technology, which can be used in seaweed cultivation, unicellular algae, botanical equipment and methods, etc., and can solve problems such as difficult cultivation environment

Inactive Publication Date: 2019-04-16
RESONAC HOLDINGS CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is known that it is difficult to maintain a culture environment such as a preferred low-light condition in the culture of this motile cell (see, for example, Non-Patent Document 1).

Method used

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  • Method for culturing photosynthetic microalgae
  • Method for culturing photosynthetic microalgae
  • Method for culturing photosynthetic microalgae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0163] Add 400ml of the medium shown in Table 1 to a 1.0L capacity flat culture flask (thickness of the flask is about 38mm including the thickness of glass), and inoculate the cystized Haematococcus NISE at 0.50g / L after autoclave sterilization -144. The astaxanthin content per unit dry mass of the inoculated Haematococcus lacustrum NISE-144 was 4.8% by mass.

[0164] Table 1

[0165] Element

g / L

KNO 3

0.7

K 2 HPO 4

0.07

MgSO 4 ·7H 2 o

0.131

CaCl 2 2H 2 o

0.063

Citric acid (anhydride)

0.0105

Ammonium iron(III) citrate

0.0105

EDTA·2Na

0.00175

Na 2 CO 3

0.035

h 3 BO 3

0.005

MnCl 2 4H 2 o

0.0032

ZnSO 4 ·7H 2 o

0.0004

Co(NO 3 ) 2 ·6H 2 o

0.000004

CuSO 4 ·5H 2 o

0.000014

(NH 4 ) 6 Mo 7 o 24 4H 2 o

0.000026

[0166]

[0167] In the case of light irradiation (light irradiatio...

Embodiment 2

[0184] Culture was carried out in the same manner as in Example 1, except that the light source for light irradiation (a) used at the start of the culture was changed from a blue LED to a white LED.

[0185] In addition, after 5 days from the start of light irradiation, the embodiment in which the light source of light irradiation (b) was changed to white LED and red LED (the ratio of light quantum flux density was 5:1) was regarded as Example 2-1, and the light source was changed to The embodiment of blue LED and red LED (the ratio of light quantum flux density is 1:1) is taken as embodiment 2-2.

[0186] Table 2 shows the type of light source, the concentration of astaxanthin in the culture solution after 12 days of culture, and the productivity of astaxanthin.

Embodiment 3

[0188]Culture was carried out in the same manner as in Example 1, except that the light source of light irradiation (a) used at the beginning of the culture was changed from blue LED to white LED and blue LED (the ratio of the flux density of photons was 5:1). .

[0189] In addition, after 5 days from the start of light irradiation, the embodiment in which the light source of light irradiation (b) was changed to white LED and red LED (the ratio of light quantum flux density was 5:1) was regarded as Example 3-1, and the light source was changed to The embodiment of blue LED and red LED (the ratio of light quantum flux density is 1:1) is taken as embodiment 3-2.

[0190] Table 2 shows the type of light source, the concentration of astaxanthin in the culture solution after 12 days of culture, and the productivity of astaxanthin.

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Abstract

The present invention provides a method for culturing photosynthetic microalgae, with which it is possible to obtain xanthophyll more efficiently compared to conventional art. This culturing method isa method for culturing photosynthetic microalgae, involving: (A) a step for increasing the number of cells by subjecting encysted photosynthetic microalgae including xanthophyll to light irradiation(a); and (B) a step for increasing the content of xanthophyll in the photosynthetic microalgae by subjecting, to light irradiation (b), the photosynthetic microalgae that has undergone the step (A) for increasing the number of cells. The light irradiation (a) and the light irradiation (b) are light irradiations employing different light sources, wherein: the light irradiation (a) is light irradiation that employs, as a light source, an LED including blue light having a wavelength from 400 to 490 nm; and the light irradiation (b) is light irradiation that employs, as a light source, an LED including blue light having a wavelength from 400 to 490 nm and an LED including red light having a wavelength from 620 to 690 nm.

Description

technical field [0001] The present invention relates to a method for cultivating photosynthetic microalgae containing lutein. Background technique [0002] Lutein is now used for various purposes. [0003] Astaxanthin, known as a type of lutein, is a type of red carotenoid and has a strong antioxidant effect. Therefore, astaxanthin is used in food coloring, cosmetics, health food, etc. [0004] Astaxanthin can also be chemically synthesized, but astaxanthin from natural sources is widely used. Natural sources of astaxanthin are extracted from shrimp such as krill and Arctic sweet shrimp, phaffia rhodozyma, algae, etc. [0005] Shrimp or Phaffia rhodozyma are known to be low in astaxanthin. Therefore, a method of obtaining astaxanthin by culturing algae has been studied. It is known that algae, such as Haematococcus genus, adapt to external environmental changes (stresses) such as depletion of nitrogen sources and strong light, and cystize, and accumulate astaxanthin in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P7/26
CPCC12N1/12C12P7/26A01G33/00C12M21/02C12M31/10
Inventor 松本阳子石仓正治铃木广志
Owner RESONAC HOLDINGS CORPORATION