Combination reagent and system for detecting acute myeloid leukemia cells
A leukemia cell and acute myeloid technology, applied in the field of combined reagents and systems for detecting acute myeloid leukemia cells, can solve problems such as low sensitivity, easy missed diagnosis, and no unified standard, meet the requirements of reducing the level of professional knowledge, and detect quickly , covering a wide range of effects
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Embodiment 1
[0081] A combined reagent for detecting acute myeloid leukemia cells, specifically as follows:
[0082] Combination 1: CD38 / CD13 / CD34 / CD117 / CD33 / CD19 / HLA-DR / CD45;
[0083] Combination 2: CD38 / CD64 / CD34 / CD123 / CD56 / CD14 / HLA-DR / CD45;
[0084] Combination 3: CD38 / CD7 / CD34 / CD5 / CD11b / CD15 / CD45.
[0085] The fluorescent labels and dosages of relevant monoclonal antibodies in the above combinations are shown in the table below.
[0086] Table 2. The dosage of each monoclonal antibody
[0087]
[0088] Note: The concentration gradient of the commercially available antibodies was verified to determine the optimal dosage, so the above-mentioned monoclonal antibodies were loaded into flow tubes numbered 1, 2, and 3, respectively.
Embodiment 2
[0090] A system for detecting acute myeloid leukemia cells, comprising: a detection module, a data acquisition module and a data analysis module.
[0091] The detection module performs flow cytometry detection on the cells to be tested;
[0092] The data acquisition module acquires the flow cytometry detection result data of the cells to be tested stained with the combined reagent described in Example 1;
[0093] The data analysis module analyzes the above acquired data, and determines whether the cells to be tested are tumor cells according to predetermined criteria.
[0094] The specific workflow of using the above system is as follows:
[0095] 1. Prepare reagents.
[0096] The combination reagents described in Example 1 were prepared.
[0097] 2. Sample processing.
[0098] The sample source of the cells to be tested can be bone marrow, peripheral blood, pleural effusion, ascites, etc., and the concentration is adjusted to 1×10 according to the number of cells. 6 -5×1...
Embodiment 3
[0125] The system for detecting acute myeloid leukemia cells in Example 2 was used to detect minimal residues of acute myeloid leukemia, and 40 daily samples were randomly selected for detection. Compared with the gold standard of bone marrow smear morphology detection, there were 0 cases of false positive results , 0 cases of false negatives, confirming that the above system can detect AML-MRD comprehensively, quickly and with high sensitivity through multi-parameter flow cytometry analysis.
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