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Preparation and detection method of fluorescent probe for iodide ion detection in water environment

A technology of fluorescent probes and iodide ions, applied in fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve problems such as time-consuming, labor-intensive, and interference

Active Publication Date: 2021-04-27
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are colorimetric methods, titration methods, electrochemical methods, chromatography, etc. for the detection of iodide ions. Although these methods can achieve sensitive and selective detection of iodide ions, most of these methods are complicated and time-consuming. , labor-intensive and many interference factors

Method used

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  • Preparation and detection method of fluorescent probe for iodide ion detection in water environment
  • Preparation and detection method of fluorescent probe for iodide ion detection in water environment
  • Preparation and detection method of fluorescent probe for iodide ion detection in water environment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Preparation of new blue-green phosphorus oxide quantum dots

[0032] Dissolve 8 mg of phosphorus pentoxide solid in 10 mL of N-methylpyrrolidone at room temperature, then add 8 mg of sodium hydroxide solid, mix and ultrasonicate for 30 minutes at room temperature, ultrasonic frequency 40KHZ, electric power 100W; The reaction solution was heated in an oil bath, and phosphorus oxide quantum dots were prepared by the solvothermal method. The temperature of the oil bath was set at 160°C, and the stirring speed was maintained at a strong speed of 360 rpm. The reaction was carried out for 8 hours; after the reaction was completed, the reaction solution was taken out The solution was centrifuged at a high speed (8000 rpm) to remove the precipitate, and the supernatant was taken; the supernatant was taken for dialysis purification, and the total time of dialysis was 2 days. The specification of the dialysis bag is 2kD. In order to make the dialysis effect good, the distille...

Embodiment 2

[0037] Prepare a phosphate buffered saline solution (PBS) with pH 6.0 in advance with deionized water for use, add 2.0 mL of buffer solution (10 mM) to a 10 mm quartz cuvette, and accurately pipette 10 μL of the pre-prepared sample obtained in Example 1 Ag + Mediated phosphorous oxide quantum dot aqueous solution (1×10 -2 mg mL -1 ) into the buffer solution in the cuvette, stirred rapidly, and put into the fluorescence meter to record the fluorescence intensity, denoted as F 0, add iodide ion (0.093mM) aqueous solution to this solution, stir for a certain period of time, until the fluorescence intensity no longer changes, record the change of fluorescence intensity under different time, such as Figure 4 As shown in (a), within 60s, the fluorescence intensity of quantum dots tends to be stable, indicating that the time period for the fluorescent probe to detect iodide ions is 1 minute, and simultaneously record different iodide ion concentrations (0.013mM, 0.021mM, 0.029 mM...

Embodiment 3

[0039] Prepare 0.02mol / L of F respectively under the conditions of Example 1 - , Cl - ,PO 4 3- ,BO 3 3- ,NO 3 2- , SO 4 2 -,NO 2 - ,Cr 2 o 7 2- (NaF, NaCl, Na 3 PO 4 , H 3 BO 3 , Na 2 SO 4 , NaNO 2 , K 2 Cr 2 o 7 ) of PBS buffer at pH 6.0, take 2 mL each and add 10 μL (1×10 -2 mg mL -1 ) of Ag + Mediated phosphorous oxide quantum dot aqueous solution, stirred and recorded fluorescence spectrum, compared with the fluorescence intensity under the action of iodide ion under the same conditions as in Example 1, obtained Figure 5 ,like Figure 5 (a), F - , Cl - ,PO 4 2- ,BO 3 3- ,NO 3 2- , SO 4 2 -,NO 2 - ,Cr 2 o 7 2- isoanions even at 200 times I - Under the condition of the concentration level, there is no fluorescence quenching ability, only Br- shows a little quenching ability, indicating that I - to Ag + mediated PO QDs have good fluorescence quenching ability, we also have the I - was tested with other co-existing anionic interfer...

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Abstract

The invention provides a method for preparing a fluorescent probe for iodide ion detection in water samples, which is characterized in that it comprises: step 1: after mixing phosphorus pentoxide solid, sodium hydroxide solid and N-methylpyrrolidone Ultrasound at room temperature, put the ultrasonic reaction solution in an oil bath, and prepare phosphorus oxide quantum dots by solvothermal method; the reaction solution is centrifuged to remove the precipitate, the supernatant is taken, and purified by dialysis, and the dialysate After concentration and freeze-drying, a blue-green fluorescent phosphorus oxide quantum dot solid material is obtained; step 2: dissolving the blue-green fluorescent phosphorus oxide quantum dot solid material in deionized water to obtain a phosphorus oxide quantum dot aqueous solution, and the phosphorus oxide quantum dot aqueous solution and Ag + Aqueous solutions are mixed to obtain Ag + The phosphorous oxide quantum dot probe mediated by the method is a fluorescent probe for the detection of iodide ions in water samples. The fluorescent probe obtained by the invention can quickly detect iodide ions, and the probe has better selectivity for iodide ions in different water samples.

Description

technical field [0001] The invention relates to a preparation method of blue-green fluorescent phosphorus oxide quantum dots and a method for detecting iodide ions, belonging to the technical field of nanomaterial preparation and application. Background technique [0002] Iodine is extremely important to animal and plant life. In advanced mammals, iodine is concentrated in the thyroid gland in the form of iodized amino acids. Iodine can promote sugar and fat metabolism, regulate water and salt metabolism, promote vitamin absorption and utilization, enhance enzyme activity, and promote growth and development. Lack of iodine can cause goiter, which seriously affects the normal development of children, manifested as mental retardation, deaf-mute, short stagnation, and growth stagnation (dwarfism). Circulatory system, digestive system, cardiovascular system and other systems, a series of hypermetabolic syndrome, hyperexcitability symptoms and eye symptoms. [0003] At present,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64C09K11/70
CPCC09K11/701G01N21/64G01N21/6428G01N21/643G01N2021/6417G01N2021/6432
Inventor 徐虎王晓梅王宇红
Owner SHANGHAI INST OF TECH
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