Promoter of map4k4 resistance gene map4k4 of bt insecticidal protein cry1ac in diamondback moth and its application

Abstract

The invention discloses a core promotor sequence for regulating expression of a plutella xylostella Bt (Bacillus Thuringiensis) Cry1Ac insecticidal protein resistant gene MAP4K4 (Mitogen-activated Protein Kinase Kinase Kinase Kinase 4), mutation sites and application of the core promotor sequence in plutella xylostella Bt Cry1Ac insecticidal protein resistance identification and field monitoring.The mutation sites are nucleotides located at a 491 site, a 474 site and 469 to 470 sites at the upstream of an initial codon ATG and are respectively named M1, M2 and M3. A nucleotide sequence of plutella xylostella Bt Cry1Ac insecticidal protein susceptible population promotor is as shown in SEQ ID NO.1, and a nucleotide sequence of a resistance near-isogenic line population promotor is as shownin SEQ ID NO.2. An experiment verifies that the activity of a MAP4K4 gene promotor is remarkably enhanced when M2 and M3 are in mutation at the same time. According to the core promotor sequence disclosed by the invention, the research of promotor mutation in the field of insect Bt resistance is promoted, and particularly, important theoretical and practical significances in aspects of insect Btresistance field detection and control are obtained.

Description

Technical field [0001] The invention relates to the field of genetic engineering, in particular to the core promoter of the MAP4K4 gene, the key gene for Bt resistance in the diamondback moth and its application. Background technique [0002] Plutella xylostella (L.), belonging to the Lepidoptera family Plutellidae, is an important agricultural pest in the world, which mainly damages cruciferous vegetables and crops. Plutella xylostella has a small individual, short generation period, and strong reproductive ability. It mainly burrows into the leaf flesh to feed on the harmful vegetables during the larval stage, forming cavities and nicks, which affect the quality and yield of vegetables. When the damage is severe, it can reduce the yield of vegetables by more than 90%, or even no harvest. At present, the annual cost of prevention and control of Plutella xylostella is as high as USD 4-50 billion worldwide. The reason why the diamondback moth can cause such serious damage is main...

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